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Invitrogen
Cdc34 Polyclonal Antibody
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Cdc34 Antibody (PA5-28173) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Cdc34 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Cdc34 Polyclonal Antibody (Product # PA5-28173) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
产品信息
PA5-28173
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the center region of human CDC34. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.61 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recommended positive controls: Raji.
Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (90%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF (FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF (SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: (E3-independent) E2 ubiquitin-conjugating enzyme R1; cell division cycle 34 homolog; E2 ubiquitin-conjugating enzyme R1; E2-CDC34; ubiquitin carrier protein; ubiquitin conjugating enzyme E2 R1; ubiquitin-conjugating enzyme Cdc34; Ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1
基因别名: AI327276; CDC34; E2-CDC34; UBC3; UBCH3; UBE2R1
UniProt ID: (Human) P49427, (Mouse) Q8CFI2
Entrez Gene ID: (Human) 997, (Mouse) 216150
protein polyubiquitination
cell cycle
protein ubiquitination
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of neuron apoptotic process
negative regulation of cAMP-mediated signaling
protein K48-linked ubiquitination
positive regulation of inclusion body assembly
G1/S transition of mitotic cell cycle
DNA replication initiation
cellular protein modification process
cellular response to interferon-beta
response to growth factor
