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CesA8, Cellulose synthase A catalytic subunit 8 (UDP-forming) Polyclonal Antibody
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CesA8, Cellulose synthase A catalytic subunit 8 (UDP-forming) Antibody (AS12-2580) in WB
500 mg of Col-0 WT Arabidopsis thaliana stem powder extracted by boiling in 2 m L of 3% SDS loading buffer + 100 m M DTT at 95 C for 10 min. Extract was spun at max speed to remove debris and supernatant was taken as crude extract. 25 µL of this was loaded on a 4-15% gel run for 50 min, 150 v. Blots were blocked with 5% milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary ... View More
产品信息
AS12-2580
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant Arabidopsis thaliana IRX1 fragment.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 100 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Cannabis sativa, Solanum lycopersicum
靶标信息
CesA8 encodes a plasma membrane-localized cellulose synthase catalytic subunit that functions in cellulose deposition during secondary cell wall formation, particularly in developing xylem, where loss of function causes characteristic irregular xylem with weakened, collapsed vessels. The CesA8 protein is a large, multi-pass membrane glycosyltransferase with an N-terminal cytosolic region containing a Cys-rich zinc-finger-like motif implicated in cellulose synthase complex assembly, followed by a central cytosolic catalytic domain typical of processive GT2 enzymes (including conserved motifs required for beta-1,4-glucan polymerization) and multiple C-terminal transmembrane helices that anchor the enzyme and support extrusion of the growing glucan chain into the cell wall. Biologically, CesA8 acts as one of the three nonredundant secondary-wall CesA isoforms that co-assemble into cellulose synthase complexes at the cell surface to synthesize cellulose microfibrils that provide mechanical strength and proper architecture to lignified secondary walls; consistent with its role in wall integrity, CesA8 disruption can also trigger compensatory stress-associated responses.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT4G18780; ATCESA8; CELLULOSE SYNTHASE 8; F28A21.190; F28A21_190; IRREGULAR XYLEM 1; IRX1; LEAF WILTING 2; LEW2
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