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Invitrogen
ChAT Polyclonal Antibody
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FIGURE: 1 / 3
ChAT Antibody (PA1-9027) in ICC/IF
Immunofluorescence analysis of ChAT was performed using Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ChAT Polyclonal Antibody (Product # PA1-9027) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Rabbit anti-Goat IgG Heavy Chain, Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27012) (1:2000 dilution) for 45 mi... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-9027
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the C terminus amino acids CKEKATRPSQGHQP (aa 736-748).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation, Antigen affinity chromatography
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, canine and porcine based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acetyl CoA; CHOACTase; Choline O-acetyltransferase
Gene Aliases: CHAT
Entrez Gene ID: (Human) 1103
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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