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Invitrogen
ChAT Polyclonal Antibody
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FIGURE: 1 / 3
ChAT Antibody (PA1-9027) in ICC/IF
Immunofluorescence analysis of ChAT was performed using Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ChAT Polyclonal Antibody (Product # PA1-9027) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Rabbit anti-Goat IgG Heavy Chain, Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27012) (1:2000 dilution) for 45 mi... View More
Product Details
PA1-9027
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
C. elegans
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the C terminus amino acids CKEKATRPSQGHQP (aa 736-748).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, canine and porcine based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acetyl CoA; acetyl CoA:choline O-acetyltransferase; CHOACTase; choline acetylase; Choline O-acetyltransferase
Gene Aliases: B230380D24Rik; CHAT; CHOACTASE; CMS1A; CMS1A2; CMS6
UniProt ID: (Human) P28329
Entrez Gene ID: (Human) 1103, (Mouse) 12647
antral ovarian follicle growth
response to hypoxia
phosphatidylcholine biosynthetic process
neurotransmitter secretion
response to nutrient
memory
acetylcholine biosynthetic process
response to drug
response to ethanol
chemical synaptic transmission
neuromuscular synaptic transmission
muscle organ development
establishment of synaptic specificity at neuromuscular junction
rhythmic behavior
adult walking behavior
dendrite development
neuron differentiation
neurotransmitter biosynthetic process
rhythmic excitation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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