Invitrogen

ChAT Polyclonal Antibody

1 篇参考文献

查看其他22个ChAT抗体

问题与答案

问题与答案

抗体检测数据 (2)

ChAT Antibody in Western Blot (WB)

ChAT Antibody in Western Blot (WB)

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ChAT Antibody (PA5-18518) in WB

Western blot was performed using Anti-ChAT Polyclonal Antibody (Product # PA5-18518) and a 74 kDa band corresponding to ChAT was observed across all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), Neuro-2a (Lane 2), IMR32 (Lane 3), Caco-2 (Lane 4), HCT-116 (Lane 5), HT-29 (Lane 6) and tissue extracts of Mouse Colon (Lane 7), Mouse Brain (Lane 8), Mouse Placenta (Lane 9), and Rat Brain (Lane 10) were electrophoresed using NuPAGE™ NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved prot... View More

ChAT Antibody in Western Blot (WB)

ChAT Antibody in Western Blot (WB)

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ChAT Antibody in Western Blot (WB) — Western blot was performed using Anti-ChAT Polyclonal Antibody (Product # PA5-18518) and a 74 kDa band corresponding to ChAT was observed across all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), Neuro-2a (Lane 2), IMR32 (Lane 3), Caco-2 (Lane 4), HCT-116 (Lane 5), HT-29 (Lane 6) and tissue extracts of Mouse Colon (Lane 7), Mouse Brain (Lane 8), Mouse Placenta (Lane 9), and Rat Brain (Lane 10) were electrophoresed using NuPAGE™ NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.75 µg/mL) and detected by chemiluminescence with Rabbit anti-Goat IgG Heavy Chain Superclonal™ Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

ChAT Antibody in Western Blot (WB) — Western blot analysis of ChAT using ChAT Polyclonal Antibody (Product # PA5-18518) (1 µg/mL) in staining of Mouse (A) and Rat (B) Brain lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

产品信息

PA5-18518

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.3-1 µg/mL

-

免疫组化 (IHC)

-

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产品规格

种属反应

Mouse, Rat

已发表种属

Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (KERARGPTKPSNLD) corresponding to the C-terminus amino acids of Chat

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.

靶标信息

ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: acetyl CoA; CHOACTase; choline acetylase; choline acetyltransferase; choline O-acetyltransferase

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基因别名: B230380D24Rik

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Entrez Gene ID: (Rat) 290567, (Mouse) 12647

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choline O-acetyltransferase activity choline binding transferase activity transferase activity, transferring acyl groups acyltransferase

antral ovarian follicle growth response to hypoxia chemical synaptic transmission neuromuscular synaptic transmission muscle organ development establishment of synaptic specificity at neuromuscular junction response to nutrient memory rhythmic behavior adult walking behavior metabolic process acetylcholine biosynthetic process dendrite development neuron differentiation neurotransmitter biosynthetic process response to drug rhythmic excitation response to ethanol