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Agrisera
ChlG (G4)/Chlorophyll synthase (chloroplastic) Polyclonal Antibody
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ChlG (G4)/Chlorophyll synthase (chloroplastic) Antibody (AS14-2793) in WB
Barley seeds were grown for 4.5 days in the dark before they were harvested and intact plastids were isolated in light according to Eichacker et al. 1996. The concentration of plastids was determined by counting in a Thoma counting chamber. The membrane complexes were isolated according to Reisinger et al. 2008 before 3 x SB buffer (2% (w/v) SDS, 10% (w/v) sucrose, 0.03% (w/v) bromophenol blue, 66 m M Na CO... View More
产品信息
AS14-2793
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana chlorophyll synthase.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Hordeum vulgare
靶标信息
ChlG encodes chlorophyll synthase, an integral membrane prenyltransferase that localizes to photosynthetic membranes (thylakoid membrane in oxygenic phototrophs and the cytoplasmic/intracytoplasmic membrane system in many bacteria), where it is positioned to couple pigment biosynthesis with assembly of chlorophyll-binding complexes. The protein belongs to the UbiA-type prenyltransferase superfamily and is a hydrophobic, multi-pass membrane enzyme whose catalytic core contains conserved Asp-rich motifs characteristic of this family that coordinate prenyl diphosphate chemistry. Functionally, ChlG catalyzes the terminal step of chlorophyll biosynthesis by esterifying chlorophyllide with a tetraprenyl alcohol moiety donated from phytyl diphosphate or geranylgeranyl diphosphate, producing (phytylated or geranylgeranylated) chlorophyll and releasing diphosphate. In cyanobacteria, ChlG is found in pigment-protein assemblies that include small high light-inducible proteins (e.g., HliD/HliC) and can associate with the YidC/Alb3 membrane insertase and the PSII biogenesis factor Ycf39, supporting a model in which ChlG not only synthesizes chlorophyll but also helps channel newly made chlorophyll toward cotranslational insertion and maturation of nascent photosystem apoproteins during acclimation and stress.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: bchG; chlG; chlg-1; G4
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