Invitrogen

Clathrin Heavy Chain Polyclonal Antibody

Name: Clathrin Heavy Chain Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-143896

查看其他30个Clathrin Heavy Chain抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (11)

Clathrin Heavy Chain Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 11

Clathrin Heavy Chain Antibody (PA5-143896) in ICC/IF

Immunocytochemistry analysis of Clathrin Heavy Chain in U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in Clathrin Heavy Chain Polyclonal antibody (Product # PA5-143896) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1... View More

Clathrin Heavy Chain Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Clathrin Heavy Chain Antibody in Western Blot (WB)

Clathrin Heavy Chain Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image Clathrin Heavy Chain Polyclonal Antibody

产品信息

PA5-143896

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.25 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Clathrin heavy chain/CLTC recombinant protein (Position: R967-Q1668).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.01mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_3075110

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human HEK293 whole cell, human Raji whole cell, rat brain tissue, rat kidney tissue, mouse brain tissue, mouse kidney tissue, mouse ANA-1 whole cell. IHC: human lung cancer tissue, mouse intestine tissue, rat intestine tissue. ICC/IF: U20S cell. Flow: U87 cell, HEPA1-6 cell, C6 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Clathrin heavy chain 1; Clathrin heavy chain 2; Clathrin heavy chain on chromosome 17; Clathrin heavy chain on chromosome 22; clathrin, heavy chain (Hc); clathrin, heavy chain-like 1; clathrin, heavy polypeptide (Hc); Clathrin, heavy polypeptide D; clathrin, heavy polypeptide-like 1; clathrin, heavy polypeptide-like 2; CLH-17; CLH-22

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基因别名: 3110065L21Rik; CHC; CHC17; CHC22; CLH-17; CLH17; CLH22; CLTC; CLTCL; CLTCL1; CLTCL2; CLTD; Hc; KIAA0034; R74732