Invitrogen

Claudin 15 Polyclonal Antibody

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26 篇参考文献

查看其他5个Claudin 15抗体

问题与答案

问题与答案

抗体检测数据 (2)
已发表图片 (11)

Claudin 15 Antibody in Immunohistochemistry (IHC)

Claudin 15 Antibody in Immunohistochemistry (IHC)

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Claudin 15 Antibody (38-9200) in IHC

Immunofluorescent co-labeling (yellow) of claudin-15 (red) and ZO-1 (green) in mouse ileum tissue using Rb anti-Claudin-15 (Product # 38-9200). Image courtesy of Jennifer Holmes and Dr. James Anderson, University of North Carolina at Chapel Hill, NC.

Claudin 15 Antibody in Immunohistochemistry (IHC)

Claudin 15 Antibody in Immunohistochemistry (IHC)

Claudin 15 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Claudin 15 Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF)

Claudin 15 Antibody in Immunohistochemistry (IHC)

Claudin 15 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Claudin 15 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Claudin 15 Antibody in Western Blot (WB)

Claudin 15 Antibody in Western Blot (WB)

Claudin 15 Antibody in Western Blot (WB)

Claudin 15 Antibody in Western Blot (WB)

Claudin 15 Antibody in Western Blot (WB)

Claudin 15 Antibody in Western Blot (WB)

Product Image Gallery

Claudin 15 Antibody in Immunohistochemistry (IHC) — Immunofluorescent co-labeling (yellow) of claudin-15 (red) and ZO-1 (green) in mouse ileum tissue using Rb anti-Claudin-15 (Product # 38-9200). Image courtesy of Jennifer Holmes and Dr. James Anderson, University of North Carolina at Chapel Hill, NC.

Claudin 15 Antibody in Immunohistochemistry (IHC) — Immunofluorescent staining of (A) Anti-Claudin 15; (B) Claudin 15 (red), ZO-1 (Green) co localization (yellow); (C) Anti-Claudin 15; (D) Claudin 15 (red), ZO-1 (green) -- C and D (blocked by fusion protein) using Rb anti-Claudin-15 (Product # 38-9200).

Claudin 15 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Segregation enables paracellular ion flux over the TJ meshwork. a Representative confocal images of MDCKII QKO cells stably expressing (sT) FLAG-Cldn2, FLAG-Cldn10a, and FLAG-Cldn2+FLAG-Cldn10a. Single claudin expressing cells were immunostained with anti-Cldn2 (magenta; 2 nd -Atto647N) or anti-Cldn10 (magenta; 2 nd -Atto647N) and anti-ZO-1 (yellow; 2 nd -AF594) antibody. Double claudin expressing cells were immunostained with anti-Cldn2 (magenta; 2 nd -Atto647N) and anti-Cldn10 (yellow; 2 nd -AF594). The white rectangle indicates the region of interest for the magnification in ( b ). b Magnification of FLAG-Cldn2+FLAG-Cldn10a expressing cells from ( a ). White arrows point out differently sized TJ parts that contain only Cldn10a. c Representative confocal images of MDCKII QKO sT FLAG-Cldn3, FLAG-Cldn15 and FLAG-Cldn3+FLAG-Cldn15. Single and double claudin expressing cells were stained with anti-Cldn3 (magenta; 2 nd -Atto647N) or anti-Cldn15 (magenta; 2 nd -Atto647N) and anti-ZO-1 (yellow; 2 nd -AF594) antibody. d Summary of the TER (ohm*cm 2 ), fluorescein flux (10 -6 cm/s) and PNa/PCl ratio from the measured cell lines: MDCKII, MDCKII QKO and MDCKII QKO sT FLAG-tagged Cldn2, Cldn3, Cldn10a, Cldn15 and MDCKII QKO sT FLAG-tagged Cldn2 + Cldn10a and FLAG-tagged Cldn3 + Cldn15. Data represent the mean +- SD from 3 to 5 independent experiments. Single data points are shown in Supplementary Fig. 12 . e Absolute permeability for sodium (PNa; magenta bars) and chloride (PCl; cyan b

Claudin 15 Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF) — Claudins form characteristic copolymers including segregation of channel-forming claudins from Cldn3. a Scheme is illustrating predicted claudin-claudin organization patterns. Two claudins (yellow and magenta) that are compatible with each other tightly colocalize in the TJ (pink). Incompatible claudins separate into different strands or even larger claudin-specific parts. This separation might facilitate permeability of specific ions (cyan) over the TJ meshwork. b Representative STED images of TJ-like meshwork of the observed five organization patterns formed by the indicated co-expressed claudins (SNAP-tagged (yellow; BG-Atto590) and YFP-tagged (magenta; alpha-GFP-NB-Atto647N)) in fixed COS-7 cells are shown. Tight colocalization: intermixing of mesh-forming claudins, integration of non-mesh-forming with a mesh-forming claudin or induction (de novo mesh-forming of co-expressed non-mesh-forming claudins). Separated claudins: segregation and exclusion. c Pearson correlation analysis of Cldn3 co-expressed with barrier-forming (gray) or channel-forming (magenta) claudins. Co-expression of Cldn3 with Cldn3 (yellow) served as positive control. Data represent the mean +- SD. Every n represents the Pearson of one TJ-like meshwork. n (Cldn3 + Cldn3) = 55; n (Cldn3 + Cldn1) = 54; n (Cldn3 + Cldn5) = 20; n (Cldn3 + Cldn6) = 20; n (Cldn3 + Cldn7) = 17; n (Cldn3 + Cldn9) = 15; n (Cldn3 + Cldn14) = 19; n (Cldn3 + Cldn19b) = 25; n (Cldn3 + Cldn2) = 36; n (Cldn3 + Cldn10a) = 30; n (Cldn3 +

Claudin 15 Antibody in Immunohistochemistry (IHC) — La-02 and C4 Improve Intestinal Permeability ( A ) IHC detection of Claudin 2 expression in colonic tissue (200X magnification); ( B ) IHC detection of Claudin 3 expression in colonic tissue (200X); ( C ) IHC detection of Claudin 15 expression in colonic tissue (200X). *represents P < 0.05, ** represents P < 0.01, *** represents P < 0.001, **** represents P < 0.0001.

Claudin 15 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — 3 FIGURE Dapiglutide promotes cation selectivity via expansion of cation-selective claudins in the epithelium. (A) Biionic dilution potentials were measured in the Ussing chamber by replacing 50% NaCl in the basal solution with counter-cation chloride in increasing size, n = 5-6 per group. Mean +- SEM, * p < 0.05 versus ICR + vehicle, SS p < 0.05 for ICR + vehicle versus ICR + dapiglutide. (B) Upper panel: representative western blot of claudin-2 protein of jejunum tissue. Lower panel: corresponding densitometric quantification, n = 4-5 per group, mean +- SEM, * p < 0.05, ** p < 0.01. (C) Upper panel: representative western blot of claudin-15 protein of jejunum tissue. Lower panel: corresponding densitometric quantification, n = 4-5 per group, mean +- SEM. (D) Immunofluorescence images of jejunum sections: claudin staining in green, F-actin in red, and nuclei in blue. Scale bar: 50 mum. White arrows indicate the extension of the indicated claudin along the crypt-villus axis

Claudin 15 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 5 Claudin-15 and ion transport are modulated in Hnf4alpha colon null mutants. (A) qRT-PCR of claudin-4, -8 and -15 at various time points in mutants (Open bars) relative to controls (Black bars). Expression levels are shown as mean values (+- SEM) relative to controls at each time point (3-14 mice per group) and were normalized with the Tbp housekeeping gene. (B) Immunoblotting confirmed modulated protein levels of claudin-4, -8 and -15 at 12 months in mutants (n = 3, open bars) and relative controls (n = 3, black bars) normalized to stable expression of E-Cadherin (Cdh1). (C) Quantification of band intensities of each claudin (means +- SEM; n = 3 or 4 for each group) relative to Cdh1. (D) Indirect immunofluorescence (FITC) of claudin-4, -8 and -15 in 3 month-old animals in proximal colon (representative of 4 pairs). Nuclei were counterstained with DAPI (blue) and merged with FITC (green). Scale bars, 50 um. (E) Claudin-15 expression is lost in the upper half of the colon epithelium in mutants (Open bar) relative to controls (Black bar). (F) Colonic permeability to 51 Cr-EDTA measured in colon tissues from 2 month-old mice (5 mice per group). (G) Baseline short circuit current (Isc) measured in colon tissues from 2 month-old mice (5 mice per group). Represented as mean values +- SEM, * P <=.05, ** P <=.01, *** P <=.001.

Claudin 15 Antibody in Western Blot (WB) — Published figure using Claudin 15 polyclonal antibody (Product # 38-9200) in Western Blot

Claudin 15 Antibody in Western Blot (WB) — Published figure using Claudin 15 polyclonal antibody (Product # 38-9200) in Western Blot

Claudin 15 Antibody in Western Blot (WB) — Published figure using Claudin 15 polyclonal antibody (Product # 38-9200) in Western Blot

Claudin 15 Antibody in Western Blot (WB) — Published figure using Claudin 15 polyclonal antibody (Product # 38-9200) in Western Blot

Claudin 15 Antibody in Western Blot (WB) — Published figure using Claudin 15 polyclonal antibody (Product # 38-9200) in Western Blot

Claudin 15 Antibody in Western Blot (WB) — Figure 6 The claudin-15 gene is a direct target of Hnf4alpha. (A) EMSA of 4 Hnf4alpha binding sites found in the 1 kb mouse claudin-15 promoter performed with 293T cells transfected with pBabe Hnf4alpha1 vector and 293T nuclear extracts as negative controls. Shifts (white arrow head) are compared to previously published APOC3 and mutated APOC3 (APOC3m) for control binding site. NS is non-specific binding (grey arrow head). Supershifts (black arrow head) were done with Hnf4alpha antibody (sc-6556). (B) ChIP assays of the 4 putative sites performed on epithelial cells from adult mouse colon. (C) qRT-PCR of claudin-15 on IEC6 cells infected with pBabe Hnf4alpha as compared to control vector pBabe (n = 4). (D) Western blot of 3 stable T84 cell populations expressing mouse claudin-15. (E) Conductance measured for three consecutive days after the reach of confluence on three populations of T84 cells expressing mouse claudin-15 or control plenti eGFP vector. Represented as mean values+-SEM, *** P <=.001.

Immunogen image

Claudin 15 Polyclonal Antibody

Immunogen Image Claudin 15 Polyclonal Antibody

产品信息

38-9200

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

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免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

-

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免疫组化（冰冻） (IHC (F))

Assay-dependent

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免疫细胞化学 (ICC/IF)

-

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

C. elegans, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the C-terminal region of the mouse Claudin-15 protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Plays a major role in tight junction specific obliteration of the intercellular space, through calcium independent cell adhesion activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Claudin-15

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基因别名: 2210009B08Rik; BB107105; CLDN15

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UniProt ID: (Human) P56746, (Mouse) Q9Z0S5, (Rat) D3ZQJ0

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Entrez Gene ID: (Human) 24146, (Mouse) 60363, (Rat) 304388

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structural molecule activity identical protein binding tight junction

ion transport calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules transport