Invitrogen

Claudin 18 Recombinant Rabbit Monoclonal Antibody (34H14L15)

Name: Claudin 18 Recombinant Rabbit Monoclonal Antibody (34H14L15)
Brand: Invitrogen
SKU: 700178

高级验证

23个已发表图片

28 篇参考文献

查看其他7个Claudin 18抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Claudin 18 Antibody in Western Blot (WB)

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Claudin 18 Antibody (700178) in WB

Western blot analysis of Claudin-18 was performed by loading 30 µg of rat Heart, A431 and A549 lysates using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. Claudin-18 was detected at ~27 kDa using Claudin-18 Recombinant Rabbit Monoclonal Antibody (... View More

Claudin 18 Antibody in Immunocytochemistry (ICC/IF)

Claudin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Claudin 18 Antibody in Immunohistochemistry (IHC)

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Immunogen image

Immunogen Image Claudin 18 Recombinant Rabbit Monoclonal Antibody (34H14L15)

产品信息

700178

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-3 µg/mL

查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

查看13篇已发表文章 13篇已发表文章

免疫组化（石蜡） (IHC (P))

2-4 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看6篇已发表文章 6篇已发表文章

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Amphibian, Dog, Human, Mouse, Pig, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

34H14L15

抗原

A peptide corresponding to amino acids 224-237 of P56856.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532290

产品详细信息

This antibody is predicted to react with chimpanzee, equine, rat and Rhesus monkey based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.The immunogenic sequence for this antibody is GFKASTGFGSNTKNH, and the antibody is predicted to react with chimpanzee, equine, rat, and Rhesus monkey based on sequence identity and similarity.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Claudin-18 is a tight junction protein expressed as lung-and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene. The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor. A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human. Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein. Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells. These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and stomach. Claudin-18 expression has also been reported in the inner ear.

仅用于科研。不用于诊断过程。未经明确授权不得转售。