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Collagen I Recombinant Rabbit Monoclonal Antibody (5F2)
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FIGURE: 1 / 11
Collagen I Antibody (MA571908) in IHC (P)
Immunohistochemistry analysis of Collagen I on paraffin-embedded Human Duodenum tissue. Antigen retrieval by boiling in sodium citrate buffer (pH 6.0) for 15 min. Blocking buffer (normal goat serum) at 37°C for 30 min. Samples were incubated with Collagen I Recombinant Monoclonal (Product # MA571908) using a dilution at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody at 37°C for... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA571908
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5F2
Immunogen
Synthetic peptide of human Collagen I between 1200 to 1300 amino acids.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha 1 (I) chain propeptide; alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha-1 collagen type 1; alpha-1 collagen type I; alpha-1 type I collagen; alpha-2 type I collagen; alpha1(I) procollagen; COL1; COL1A1; collagen 1 pro-alpha-2 chain; collagen alpha 1 chain type I; collagen alpha-1(I) chain preproprotein; collagen Col1-ColIII-1; collagen Col1-ColIII-2; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, type I, alpha 1; collagen, type I, alpha 2; epididymis secretory sperm binding protein; pre-pro-alpha-2 type I collagen; pro-alpha-1 collagen type 1; Type I Collagen; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain; unnamed protein product
Gene Aliases: CAFYD; EDSARTH1; EDSARTH2; EDSC; EDSCV; OI1; OI2; OI3; OI4
UniProt ID: (Human) P02452, (Human) P08123
Entrez Gene ID: (Human) 1277, (Human) 1278
skeletal system development
blood vessel development
transforming growth factor beta receptor signaling pathway
Rho protein signal transduction
regulation of blood pressure
collagen fibril organization
odontogenesis
skin development
skin morphogenesis
animal organ development
ossification
osteoblast differentiation
response to nutrient
visual perception
sensory perception of sound
response to xenobiotic stimulus
response to mechanical stimulus
positive regulation of epithelial to mesenchymal transition
positive regulation of cell migration
response to nutrient levels
response to estradiol
response to insulin
collagen biosynthetic process
protein localization to nucleus
tooth mineralization
response to hydrogen peroxide
response to peptide hormone
cellular response to fibroblast growth factor stimulus
positive regulation of transcription, DNA-templated
response to steroid hormone
embryonic skeletal system development
system development
response to cAMP
response to hyperoxia
cellular response to mechanical stimulus
cellular response to retinoic acid
cellular response to vitamin E
cellular response to glucose stimulus
cellular response to tumor necrosis factor
cellular response to epidermal growth factor stimulus
cellular response to transforming growth factor beta stimulus
positive regulation of canonical Wnt signaling pathway
response to fluoride
cellular response to fluoride
cellular response to acetaldehyde
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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