Invitrogen

Collagen II Polyclonal Antibody

Name: Collagen II Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-95138

查看其他24个Collagen II抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

Collagen II Antibody in Western Blot (WB)

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Collagen II Antibody (PA5-95138) in WB

Western blot analysis of Collagen II in Lane 1: COLO320 whole cell lysate (40 µg), Lane 2: HeLa whole cell lysate (40 µg), Lane 3: MCF-7whole cell lysate (40 µg), Lane 4: A549 whole cell lysate (40 µg), Lane 5: Jurkat whole cell lysate (40 µg). Samples were incubated with Collagen II polyclonal antibody (Product # PA5-95138).

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Immunogen image

Immunogen Image Collagen II Polyclonal Antibody

产品信息

PA5-95138

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen II (1222-1241aa AFAGLGPREKGPDPLQYMRA).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide, 0.05mg thimerosal

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2806943

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: COLO320 whole cell, HELA whole cell, MCF-7whole cell, A549 whole cell, JURKAT whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

COL2A1 is the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Alpha-1 type II collagen; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; CGA; chondrocalcin; Collagen 2; Collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen type II (Col2A1) gene, enhancer region; collagen, type II, alpha 1; disproportionate micromelia; MGC131516; Procollagen II alpha 1; procollagen, type II, alpha 1

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基因别名: ANFH; AOM; CG2A1A; COL11A3; Col2; Col2a; Col2a-1; COL2A1; COLLII; Del1; Dmm; Lpk; M100856; Rgsc856; SEDC; STL1

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UniProt ID: (Human) P02458, (Rat) P05539, (Mouse) P28481

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Entrez Gene ID: (Human) 1280, (Rat) 25412, (Mouse) 12824

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功能

extracellular matrix structural constituent conferring tensile strength identical protein binding metal ion binding platelet-derived growth factor binding extracellular matrix structural constituent extracellular matrix structural protein

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