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          • Primary Antibodies ›
          • Collagen IV Antibodies

          Invitrogen

          Collagen IV Polyclonal Antibody

          1 Published Figure
          5 References
          View all (32) Collagen IV antibodies

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          Cite Collagen IV Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          • Published Figures (1)
          Collagen IV Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          Collagen IV Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Collagen IV Antibody (PA1-28534) in IHC (P)

          Immunohistochemistry analysis of Collagen IV was performed in human kidney, glomeruli and liver tissues using Collagen IV Polyclonal Antibody (Product # PA1-28534) at a dilution of 1:400 for 45 minutes a room temperature. The antibody showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Staining for... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Collagen IV Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Collagen IV Antibody in Immunohistochemistry (IHC)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Collagen IV Polyclonal Antibody

          Product Details

          PA1-28534

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:10,000
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Multiplex Immunohistochemistry (mIHC)

          Assay-dependent
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          1:100
          View 1 publication 1 publication

          Dot blot (DB)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Bovine, Human

          Published species

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Collagen Type IV from human and bovine placenta.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.18 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.01% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1956972

          Product Specific Information

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          Collagen IV is a type of collagen protein that is a major component of the basement membrane in various tissues, including the kidneys, lungs, and skin. It forms a network-like structure that provides support and stability to the surrounding tissue. Collagen IV is composed of three alpha chains, which are encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes can lead to various genetic disorders, such as Alport syndrome and Goodpasture syndrome, which affect the kidneys and lungs, respectively.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; Collagen alpha-6(IV) chain; collagen, type IV, alpha 1; collagen, type IV, alpha 3 (Goodpasture antigen); Goodpasture antigen; Type IV Collagen

          View more View less

          Gene Aliases: COL4A1; COL4A2; COL4A3; COL4A4; COL4A5; COL4A6

          View more View less

          UniProt ID: (Bovine) Q7SIB2

          View more View less

          Entrez Gene ID: (Bovine) 282191, (Bovine) 317711, (Bovine) 407107

          View more View less

          Function(s)
          extracellular matrix structural constituent conferring tensile strength platelet-derived growth factor binding extracellular matrix structural protein
          Process(es)
          patterning of blood vessels brain development neuromuscular junction development collagen-activated tyrosine kinase receptor signaling pathway retinal blood vessel morphogenesis renal tubule morphogenesis cellular response to amino acid stimulus basement membrane organization cell adhesion
          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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