Search

  • Contact Us
  • Quick Order

Rockland

Collagen Type I Polyclonal Antibody, HRP

View all (14) Collagen I antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
Collagen Type I Polyclonal Antibody, HRP

Product Details

600-403-103

Applications
Tested Dilution
Publications

Western Blot (WB)

1:3,000-1:6,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Flow Cytometry (Flow)

5 µg/mL
-

ELISA (ELISA)

1:3,000-1:6,000
-

Immunoprecipitation (IP)

1:100
-
Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Collagen Type I from human and bovine placenta.
3D Epitope / Immunogen

Conjugate

HRP HRP HRP

Form

Lyophilized

Concentration

1 mg/mL

Amount

50 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 10mg/mL BSA

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Target Information

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: alpha-1 type 1 collagen; Alpha-1 type I collagen; Alpha-2 type I collagen; COL1; collagen 1 alpha 2 chain; Collagen alpha-1(I) chain; Collagen alpha-2(I) chain; collagen COL1A2; collagen, type 1, alpha 1; collagen, type I, alpha 1; collagen, type I, alpha 2; osteogenesis imperfecta; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; procollagen, type I, alpha 2; Type I Collagen; type I-alpha 1 collagen

View more View less

Gene Aliases: AA960264; AI325291; Col1a-1; Col1a-2; COL1A1; COL1A2; Cola-1; Cola-2; Cola1; Cola2; COLIA1; Mov-13; Mov13; oim

View more View less

UniProt ID: (Bovine) Q3MHM2, (Bovine) P02465, (Rat) P02454, (Mouse) P11087, (Mouse) Q01149, (Rat) P02466

View more View less

Entrez Gene ID: (Bovine) 282187, (Bovine) 282188, (Rat) 29393, (Mouse) 12842, (Mouse) 12843, (Rat) 84352

View more View less

Function(s)
protease binding protein binding identical protein binding metal ion binding platelet-derived growth factor binding protein binding, bridging SMAD binding molecular_function extracellular matrix structural constituent extracellular matrix structural protein
Process(es)
osteoblast differentiation intramembranous ossification endochondral ossification visual perception sensory perception of sound positive regulation of epithelial to mesenchymal transition negative regulation of cell-substrate adhesion protein transport collagen fibril organization positive regulation of cell migration collagen biosynthetic process protein localization to nucleus tooth mineralization skin morphogenesis positive regulation of transcription, DNA-templated embryonic skeletal system development face morphogenesis bone trabecula formation cartilage development involved in endochondral bone morphogenesis cellular response to amino acid stimulus cellular response to mechanical stimulus positive regulation of canonical Wnt signaling pathway skeletal system development blood vessel development transforming growth factor beta receptor signaling pathway Rho protein signal transduction regulation of blood pressure bone mineralization collagen metabolic process protein heterotrimerization extracellular matrix assembly ossification response to nutrient response to mechanical stimulus response to nutrient levels response to corticosteroid response to estradiol wound healing response to drug response to hydrogen peroxide response to peptide hormone skin development cellular response to fibroblast growth factor stimulus tooth eruption response to steroid hormone skeletal system morphogenesis response to cAMP response to hyperoxia cellular response to retinoic acid cellular response to vitamin E cellular response to tumor necrosis factor cellular response to epidermal growth factor stimulus cellular response to transforming growth factor beta stimulus response to fluoride cellular response to fluoride cellular response to organic substance response to norepinephrine cellular response to thyroid hormone stimulus
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support