Rockland

Collagen Type IV Polyclonal Antibody

Name: Collagen Type IV Polyclonal Antibody
Brand: Rockland
SKU: 600-101-MN4

查看其他30个Collagen IV抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

Collagen Type IV Antibody in Immunocytochemistry (ICC/IF)

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Collagen Type IV Antibody (600-101-MN4) in ICC/IF

Immunofluorescence of Goat Anti-Collagen Type IV Antibody. Cells: NIH/3T3 cells. Fixative: 4%PFA. Permeabilization: None. Primary Antibody: Anti-Collagen Type IV at 15µg/mL overnight at 2-8°C. Counterstain: Donkey Anti-Goat IgG Dylight™488 (p/n 605-741-125) at 5µg/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-Collagen Tye IV + Dylight™488. (C) Merge A+B. (D) secondary only... View More

Collagen Type IV Antibody in Immunohistochemistry (IHC)

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Immunogen image

Immunogen Image Collagen Type IV Polyclonal Antibody

产品信息

600-101-MN4

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

免疫组化 (IHC)

1:100

免疫细胞化学 (ICC/IF)

15 µg/mL

酶联免疫吸附实验 (ELISA)

1:10,000-1:50,000

产品规格

种属反应

Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Anti-Collagen IV antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 240-265 of mouse Collagen IV.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.92 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against mouse Collagen IV. Blast analysis of the sequence of the immunogen shows 100% identity with mouse and 78.6% identity with human.

靶标信息

Collagen IV is a type of collagen protein that is a major component of the basement membrane in various tissues, including the kidneys, lungs, and skin. It forms a network-like structure that provides support and stability to the surrounding tissue. Collagen IV is composed of three alpha chains, which are encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes can lead to various genetic disorders, such as Alport syndrome and Goodpasture syndrome, which affect the kidneys and lungs, respectively.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: alpha1(IV) collagen; canstatin; col4a3; col4a4; Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; collagen alpha-5(IV) chain; collagen alpha-6(IV) chain; collagen type IV alpha3 chain; collagen type IV alpha5 chain; Del(8)Bru44H; procollagen type IV alpha 5; procollagen type IV alpha 6; procollagen, type IV, alpha 1; procollagen, type IV, alpha 2; procollagen, type IV, alpha 3; procollagen, type IV, alpha 4; procollagen, type IV, alpha 5; procollagen, type IV, alpha 6; retinal anterior wiring; tumstatin; Type IV Collagen

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基因别名: [a]3(IV); [a]4(IV); alpha3(IV); BB116301; Bru; Col4a-1; Col4a-2; Col4a1; Col4a2; Col4a3; Col4a4; Del(8)44H; E130010M05Rik; Raw; Svc