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          • Primary Antibodies ›
          • COL6A1 Antibodies

          Rockland

          Collagen Type VI Polyclonal Antibody, Biotin

          查看其他12个COL6A1抗体

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          Cite Collagen Type VI Polyclonal Antibody, Biotin

          Additional Information:
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          • 抗体检测数据 (1)
          Collagen Type VI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Collagen Type VI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          图: 1 / 1

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          Collagen Type VI Antibody (600-406-108) in IHC (P)

          Immunohistochemistry of Rabbit anti-collagen VI antibody. Tissue: human placenta (left), testis (right). Fixation: formalin fixed paraffin embedded. Antigen retrieval: steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Primary antibody: collagen VI antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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          Collagen Type VI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Collagen Type VI Polyclonal Antibody, Biotin

          产品信息

          600-406-108

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:5,000-1:10,000
          -

          免疫组化(石蜡) (IHC (P))

          1:200-1:1,000
          -

          酶联免疫吸附实验 (ELISA)

          1:10,000-1:50,000
          -

          免疫沉淀 (IP)

          1:100
          -
          产品规格

          种属反应

          Bovine, Human

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          Collagen Type VI from human and bovine placenta
          查看免疫原

          偶联物

          Biotin Biotin Biotin

          形式

          Lyophilized

          浓度

          1 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA

          内含物

          0.01% sodium azide

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          运输条件

          Ambient (domestic); Wet ice (international)

          产品详细信息

          Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

          Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

          靶标信息

          The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (typesI, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

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          引用该产品

          生物信息学

          蛋白别名: alpha 1 (VI) chain (61 AA); Collagen alpha-1(VI) chain; collagen VI, alpha-1 polypeptide; collagen, type VI, alpha 1; epididymis secretory sperm binding protein; unnamed protein product

          查看更多 收起

          基因别名: BTHLM1; BTHLM1A; COL6A1; OPLL; UCHMD1; UCHMD1A

          查看更多 收起

          UniProt ID: (Human) P12109

          查看更多 收起

          Entrez Gene ID: (Human) 1291, (Bovine) 511422

          查看更多 收起

          功能
          protein binding collagen binding extracellular matrix structural constituent conferring tensile strength platelet-derived growth factor binding extracellular matrix structural protein
          参与通路
          response to reactive oxygen species cell morphogenesis ossification osteoblast differentiation hair follicle development reduction of food intake in response to dietary excess muscle system process respiratory system process glycolytic process tricarboxylic acid cycle 2-oxoglutarate metabolic process energy reserve metabolic process autophagy apoptotic process inflammatory response mitochondrion organization cell adhesion single fertilization peripheral nervous system development heart development muscle organ development skeletal muscle tissue development circadian rhythm insulin receptor signaling pathway regulation of cell size response to xenobiotic stimulus response to UV response to wounding response to mechanical stimulus response to toxic substance anatomical structure morphogenesis tissue development gene expression muscle cell apoptotic process response to activity response to muscle activity transmission of nerve impulse myelination in peripheral nervous system cell differentiation collagen fibril organization apoptotic nuclear changes bone mineralization lung development response to lipopolysaccharide collagen metabolic process response to lipid endodermal cell differentiation limb joint morphogenesis response to decreased oxygen levels skeletal muscle tissue regeneration protein kinase B signaling skin development insulin-like growth factor receptor signaling pathway response to pain lung alveolus development skeletal muscle tissue growth skeletal muscle fiber development tissue remodeling homeostasis of number of cells neurological system process sensory perception of mechanical stimulus cartilage development protein tetramerization neuron apoptotic process mitochondrial depolarization muscle cell development uterus development canonical Wnt signaling pathway bone development lung morphogenesis lung epithelial cell differentiation skeletal muscle organ development adipose tissue development muscle structure development fat cell proliferation caveola assembly cellular response to amino acid stimulus basement membrane organization multicellular organismal locomotion reactive oxygen species metabolic process extracellular matrix assembly energy homeostasis skeletal muscle fiber differentiation response to peptide regulation of collagen fibril organization response to polyamine macromolecule response to bleomycin mitochondrial transmembrane transport
          It has to be done as per old AB suggested Products section.

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