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Invitrogen
Creatine Kinase BB Monoclonal Antibody (CK-CYK/21E10)
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产品信息
MA5-33339
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rabbit,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
CK-CYK/21E10
抗原
Synthetic 17-mer peptide corresponding to the N-terminal sequence of human creatine kinase brain-type (SNSHNALKLRFPAEDEF)
偶联物
形式
Liquid
浓度
100 µg/mL
纯化类型
Protein G
保存液
PBS with 0.1% BSA
内含物
0.02% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Product is supplied at 1 mL (100 µL/mL). Application notes: Western blot - Under reducing conditions, antibody detected a protein with a band size of 43-46 kDa. IHC-F- tissue was fixed for 2 hours in 2% paraformaldehyde and 0.1 % glutaraldehyde in 0.1 M PB. The tissue was stored until use in 1 % paraformaldehyde. The tissue was blocked with 1% normal swine serum. IHC-P - antigen retrieval: 0.5 % pepsin in 0.01 N HCl for 15 min at 37 °C; endogenous peroxidise blocking by using 3 % H2O2 in PBS for 30 min; blocking with 1% normal swine serum for 30 min.; Purkinje cells serve as positive control and connective tissue as negative control.
Positive control: Brain lysate, Human Purknije cells; Negative control: Human fibroblasts
靶标信息
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: B-CK; Brain creatine kinase; CPK-B; creatine kinase B; Creatine kinase B chain; Creatine kinase B-type; creatine kinase brain; creatine kinase brain-type; creatine kinase, brain; creatine kinase-B; Creatine phosphokinase B-type; Creatine phosphokinase M-type; epididymis luminal protein 211; epididymis secretory protein Li 29
基因别名: B-CK; BCK; Ck-3; Ck3; CKB; CKBB; Ckbr; CPK-B; HEL-211; HEL-S-29
UniProt ID: (Human) P12277, (Rabbit) P00567, (Rat) P07335, (Mouse) Q04447
Entrez Gene ID: (Human) 1152, (Rabbit) 100009085, (Rat) 24264, (Mouse) 12709
