Invitrogen

Cullin 2 Polyclonal Antibody

Name: Cullin 2 Polyclonal Antibody
Brand: Invitrogen
SKU: 51-1800

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36个已发表图片

33 篇参考文献

查看其他11个Cullin 2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Cullin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Cullin 2 Antibody (51-1800) in IHC (P)

Immunohistochemistry analysis of CUL-2 showing staining in the cytoplasm and nucleus of paraffin-embedded human Ovarian carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min ... View More

Cullin 2 Antibody in Immunoprecipitation (IP)

Cullin 2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Immunogen image

Immunogen Image Cullin 2 Polyclonal Antibody

产品信息

51-1800

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:250-1:1,000

查看25篇已发表文章 25篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:50

酶联免疫吸附实验 (ELISA)

0.1-1.0 µg/mL

免疫沉淀 (IP)

2-3 µg/mL

查看4篇已发表文章 4篇已发表文章

亲和纯化 (AP)

查看1篇已发表文章 1篇已发表文章

GST融合蛋白沉降技术 (GPD)

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其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

C. elegans, Human, Insect, Mouse, Tag

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the C-terminus of the human CUL2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533898

靶标信息

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。