Invitrogen

Cullin 4A/4B Recombinant Rabbit Monoclonal Antibody (K01_1P95)

Name: Cullin 4A\/4B Recombinant Rabbit Monoclonal Antibody (K01_1P95)
Brand: Invitrogen
SKU: MA567853

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (2)

Cullin 4A/4B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 2

Cullin 4A/4B Antibody (MA567853) in IHC (P)

Immunohistochemical (Paraffin) analysis of Cullin 4A/4B in Human lung cancer using Cullin 4A/4B Recombinant Monoclonal Antibody (Product # MA567853). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Cullin 4A/4B Antibody in Western Blot (WB)

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产品信息

MA567853

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化（石蜡） (IHC (P))

1:50-1:100

产品规格

种属反应

Hamster, Human, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293F

分类

Recombinant Monoclonal

类型

Antibody

克隆号

K01_1P95

抗原

A synthetic peptide of human Cullin 4B.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Protein A/G

保存液

50mM tris glycine, pH 7.4, with 0.05% BSA, 150mM NaCl, 40% glycerol

内含物

0.01% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Cullin 4A and Cullin 4B, encoded by CUL4A and CUL4B, are scaffold proteins that assemble cullin RING E3 ubiquitin ligase complexes known as CRL4. These complexes typically pair CUL4A or CUL4B with the adaptor DDB1 and diverse DCAF substrate receptors to target numerous proteins for ubiquitination, thereby controlling DNA replication licensing through CDT1 turnover, nucleotide excision repair via regulation of DDB2 and XPC, chromatin dynamics, cell cycle progression, and signal transduction. CUL4A is frequently amplified or overexpressed in several cancers and has been linked to increased proliferation and therapy resistance, while CUL4B mutations cause an X linked intellectual disability syndrome with developmental anomalies, reflecting nonredundant functions and distinct subcellular localization patterns. CRL4 activity is regulated by NEDD8 conjugation known as neddylation, by deneddylation through the COP9 signalosome, and by availability of DCAF receptors, making the pathway sensitive to small molecule neddylation inhibitors such as MLN4924 also known as pevonedistat. Experimental interrogation of CUL4 biology includes proteomics to map DCAF interactions, ubiquitinome profiling to define substrates, and genetic perturbation to dissect isoform specific roles in genome integrity and oncogenesis.

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