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Invitrogen
Cutaneous Lymphocyte Antigen (CLA) Monoclonal Antibody (HECA-452), eBioscience™
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Cutaneous Lymphocyte Antigen (CLA) Antibody (14-9857-82) in IHC (P)
Immunohistochemistry on formalin-fixed paraffin embedded human tonsil, using 10 µg/mL Rat IgM Isotype Control (left) or 10 µg/mL Anti-Human/Mouse Cutaneous Lymphocyte Antigen (CLA) Purified (right) followed by biotinylated anti-rat IgG and DAB visualization.Nuclei are counterstained with hematoxylin.
产品信息
14-9857-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rat
/ IgM, kappa
分类
Monoclonal
类型
Antibody
克隆号
HECA-452
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: This HECA-452 monoclonal antibody reacts with human Cutaneous Lymphocyte Antigen (CLA). This cell surface glycoprotein is most highly expressed on T cells in the skin. Nevertheless, CLA can also be detected on some peripheral T and NK cells, as well as monocytes, neutrophils and CD1a+ dendritic cells. Studies have determined that this antibody recognizes sialofucosylated glycans and sialyl Lewis x (sLex). CLA, which binds E-selectin and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1), is involved in T lymphocyte infiltration and adhesion at cutaneous sites of inflammation.
The HECA-452 antibody has been reported to inhibit sLex binding to E-selectin; however, this property is still under debate in the literature.
Applications Reported: This HECA-452 antibody has been reported for use in flow cytometric analysis, western blotting, functional assays, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.
Applications Tested: This HECA-452 antibody has been tested by flow cytometric analysis on human PBMCs and by immunohistochemistry on FFPE human tissue. The HECA-452 antibody can be used at less than or equal to 0.125 µg/test for flow cytometry and at less than or equal to 10 µg/mL. The antibody performs optimally with low pH antigen retrieval but high pH antigen retrieval can also be used. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
This cell surface glycoprotein is most highly expressed on T cells in the skin. Nevertheless, CLA can also be detected on some peripheral T and NK cells, as well as monocytes, neutrophils and CD1a+ dendritic cells. Studies have determined that this antibody recognizes sialofucosylated glycans and sialyl Lewis x (sLex). CLA, which binds E-selectin and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1), is involved in T lymphocyte infiltration and adhesion at cutaneous sites of inflammation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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