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Rockland
Cyanine Polyclonal Antibody
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FIGURE: 1 / 1
Cyanine Antibody (600-601-209) in ELISA
ELISA of results purified Sheep anti-Cyanine Antibody. Each well was coated in duplicate with 1.0 µg of BSA-conjugated Cy5. The starting dilution of antibody was 5 µg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using blocking buffer MB-060-1000, Donkey anti-Sheep HRP conjugate 1:10,000, and TMB-8000 substrate.
Product Details
600-601-209
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A mixture of Cy3 and Cy5 conjugated KLH
Conjugate
Form
Liquid
Concentration
1.1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at 2-8° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown.
Target Information
Cyanines are blue-green fluorescent dyes belonging to polymethine group, used in biotechnology for labeling and analysis. The spectrum for Cyanine ranges from IR (489/506) to UV (743/767). Cyanine dyes include Cy2, Cy3, Cy3B, Cy3.5, Cy5, Cy5.5 and Cy7. These dyes exhibit brighter and more stable fluorescence than traditional FITC, TRITC/Rhodamine dyes. Cy3 and Cy5 are most commonly used for 2 color detection in Flow Cytometry applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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