Invitrogen

Cyclin A2 Monoclonal Antibody (E23.1)

Name: Cyclin A2 Monoclonal Antibody (E23.1)
Brand: Invitrogen
SKU: MA1-154

高级验证

3个已发表图片

6 篇参考文献

查看其他28个Cyclin A2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Cyclin A2 Antibody in Immunocytochemistry (ICC/IF)

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Cyclin A2 Antibody (MA1-154) in ICC/IF

Immunofluorescence analysis of Cyclin A2 was performed using 70% confluent log phase HeLa cells treated with Camptothecin (100nM, 16 hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cyclin A2 Monoclonal Antibody (Product # MA1-154) at 1:50 dilution in 0... View More

Cyclin A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cyclin A2 Antibody in Immunoprecipitation (IP)

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产品信息

MA1-154

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看6篇已发表文章 6篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:500

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:50

免疫沉淀 (IP)

1 µg

产品规格

种属反应

Bovine, Human, Mouse, Non-human primate, Rat

已发表种属

Human, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

E23.1

抗原

Bovine Cyclin A fusion protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2536862

产品详细信息

MA1-154 detects Cyclin A in mammalian samples and has been successfully used in Immunohistochemistry (Frozen and Paraffin), Immunofluorescence, Immunoprecipitation and Western Blot procedures. Immunoprecipitation and Western Blot analysis with MA1-154 show the accumulation of a prominent band at ~50 kDa in camptothecin and hydroxyurea treated cells. In Immunofluorescence applications, MA1-154 shows nuclear translocation of Cyclin A in G2/M arrested cells as a result of camptothecin treatment.

靶标信息

Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin / cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S phase and passage through G2-phase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。