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Cyt f/Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (higher plants) Polyclonal Antibody
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Cyt f/Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (higher plants) Antibody (AS20-4377) in WB
Samples: 1 - 25 µg of Picea abies total proteins extract obtained from needles using 10% TCA/acetone, pellet was dissolved in 8 M Urea, 40 m M Tris-HCl, p H 6.8,0,1 m M EDTA, 1% SDS 2 - 25 µg of Pinus sylvestris total proteins extract obtained from needles using 10% TCA/acetone, pellet was dissolved in 8 M Urea, 40 m M Tris-HCl, p H 6.8,0,1 m M EDTA, 1% SDS 3 - 10 µg of Lupinus angustifolius thylakoid membr... View More
产品信息
AS20-4377
产品规格
种属反应
Bacteria,
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana PetA sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Cucumis sativus, Echinola crus-galli, Ficus elastica, Lupinus angustifolius, Nicotiana tabacum, Phaseolus coccineus , Picea abies, Pinus sylvestris, Pisum sativum, Solanum tuberosum, Synechococcus elongatus PCC7942, Zea mays
靶标信息
Cyt f is the c-type cytochrome subunit of the photosynthetic cytochrome b6f complex, encoded by the chloroplast petA gene and integrated into the thylakoid membrane with a single C-terminal transmembrane helix that anchors a large hydrophilic domain on the lumenal side. The mature protein binds a high-potential c-type heme via the canonical Cys-X2-Cys-His motif, forming a predominantly beta-sheet-rich extrinsic (lumen-facing) domain with an unusual heme iron axial ligation involving the N-terminus, as revealed by X-ray crystallography. Functionally, Cyt f accepts electrons from the Rieske 2Fe-2S protein within cytochrome b6f and donates them to plastocyanin in the thylakoid lumen, thereby supporting intersystem electron transfer between photosystem II and photosystem I; this process is coupled, at the complex level, to proton translocation that contributes to the proton motive force driving ATP synthesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cyt f; cytochrome f; petA
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