Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Cytochrome C Monoclonal Antibody (2B5), eBioscience™
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
图: 1 / 4
Cytochrome C Antibody (BMS1037) in ICC/IF
Immunofluorescence analysis of Cytochrome C was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cytochrome C Monoclonal Antibody (2B5), eBioscience™ (Product # BMS1037) at 1:100 dilution in 0.1% BSA, ... View More
产品信息
BMS1037
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
2B5
抗原
Rec. Human Cytochrome C
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2-7.4
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: Cytochrome c was identified as a component required for the crucial steps in apoptosis, caspase-3 activation and DNA fragmentation. Cytochrome c was shown to redistribute from mitochondria to cytosol during apoptosis in intact cells.
Mitochrondrial cytochrome c is a water-soluble protein of 15 kDa with a net positive charge, residing loosely attached in the mitochrondrial intermembrane space. Cytochrome c functions in the respiratory chain by interaction with redox partners. The release of cytochrome c into the cytosol leads to an activation of an apoptotic program via activation of a caspase dependent pathway. Cytochrome c achieves this goal by interaction with other cytosolic factors forming a complex (apoptosome) composed of cytochrome c, Apaf-1, dATP and Apaf-3/caspase 9. Bcl-2 on the other hand was shown to be able to prevent apoptosis by blocking the release of cytochrome c from mitochondria.
Applications Tested: ELISA, Flow Cytometry, Western Blotting.
靶标信息
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CRF; Cytochrome c; hc1
基因别名: CYC; CYCS; HCS; THC4
UniProt ID: (Human) P99999
Entrez Gene ID: (Human) 54205
response to reactive oxygen species
mitochondrial electron transport, ubiquinol to cytochrome c
mitochondrial electron transport, cytochrome c to oxygen
protein dephosphorylation
mitochondrion organization
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
cellular respiration
intrinsic apoptotic signaling pathway
