Invitrogen

Cytochrome C Monoclonal Antibody (2G8), eBioscience™

Name: Cytochrome C Monoclonal Antibody (2G8), eBioscience\u2122
Brand: Invitrogen
SKU: BMS195

2 篇参考文献

查看其他50个Cytochrome C抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

产品信息

BMS195

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

查看2篇已发表文章 2篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-Dependent

功能检测 (Functional)

Assay-Dependent

产品规格

种属反应

Human

已发表种属

Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

2G8

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10609203

产品详细信息

Description: A second major function for cytochrome c is its role as an intermediate in apoptosis, both through its association with inositol-3-phosphate receptor (IP3 receptor) which acts as an ER calcium release channel, and its association with apoptotic protease activating factor 1 (Apaf-1), resulting in the formation of the apoptosome protein complex which then recruits and activates pro-caspase 9 (Apaf-3).

This antibody recognises cytochrome c, originally termed Apaf-2, an evolutionarily conserved protein which acts as an essential mitochondrial electron carrier between respirable substrates and oxygen, during aerobic energy production. Clone 2G8 is reported to block the role of cytochrome c as a co-factor in the activation of caspase 9.

Applications Tested: ELISA, Flow Cytometry (Intracellular Staining), Functional Studies (Blocking).

靶标信息

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。