Invitrogen

Cytochrome C Antibody Cocktail

Name: Cytochrome C Antibody Cocktail
Brand: Invitrogen
SKU: 459160

1 篇参考文献

查看其他50个Cytochrome C抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

Cytochrome C Antibody in Western Blot (WB)

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Cytochrome C Antibody (459160) in WB

Western blot analysis of Cytochrome C in Jurkat, 143B, and HeLa cells using a Cytocrome C Monoclonal antibody (Product # 459160). Apoptosis was induced in Jurkat, 143B osteosarcoma, and HeLa cells bia treatment with FAS and staurosporine. Isolation of Mitochondrial and cytoplasmic fractions were performed before detecting with the Cyctocrome C Monoclonal antibody (Product # 459160). The western blot shows that Cytochrome c has translocated partially in FAS-induced cells and STS-treated 43B osteosarcoma cells, and almost completely in STS-t... View More

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产品信息

459160

应用

建议稀释比

已发表文章

免疫印迹 (WB)

3.6 µg/mL

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / Ig

分类

Cocktail

类型

Antibody

克隆号

Cocktail

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.9 mg/mL

纯化类型

IgG fraction

保存液

HEPES buffered saline

内含物

0.02% sodium azide

保存条件

4°C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_2532229

产品详细信息

ApoTrack™ Cytochrome c WB Cocktail (Sku# 459160) provides a means to detect apoptosis while detecting release of cytochrome c, as well as a cytoplasmic and mitochondrial markers with the help of western blot. This cocktail provides a means to detect cytochrome c, as well as a cytoplasmic and mitochondrial markers

Antibodies Cocktail Contains:

Mouse anti Cyt. c monoclonal:

Amount: 50 µg

Working concentration: 1 µg/mL

Mouse anti GAPDH monclonal:

Amount: 5 µg

Working concentration: 0.1 µg/mL

Mouse anti PDH-E1-alpha monoclonal:

Amount: 100 µg

Working concentration: 2 µg/mL

Mouse anti C-V-alpha monoclonal:

Amount: 25 µg

Working concentration: 0.5 µg/mL

靶标信息

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。