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Invitrogen
Cytokeratin 19 Monoclonal Antibody (RCK108)
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Cytokeratin 19 Antibody (MA1-06329) in ICC/IF
Immunofluorescence analysis of Cytokeratin 19 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 19 Mouse Monoclonal Antibody (RCK108) (Product # MA1-06329) at 5 µg/mL in 0.1% BSA, incubated at... View More
产品信息
MA1-06329
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Zebrafish
已发表种属
C. elegans,
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
RCK108
抗原
Cytoskeletal preparation of human bladder carcinoma cell line T24.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
purified
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1-06329 detects cytokeratin 19 in human and zebrafish samples.
MA1-06329 has sucessfully been used in Western blotting, flow cytometry, immunocytochemistry and immunohistochemistry.
The MA1-06329 immunogen is a cytoskeletal preparation of human bladder carcinoma cell line T24.
靶标信息
Cytokeratin 19 is part of a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight from 40-68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. For example, cytokeratin 19 is not expressed in hepatocytes, therefore, it is useful in the identification of liver metastasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 40-kDa keratin intermediate filament; C K1CS; CK 19; CK-19; cytokeratin 19; Cytokeratin-19; K19; keratin 19, type I; Keratin, type I cytoskeletal 19; keratin, type I, 40-kd; Keratin-19; MGC15366
基因别名: CK19; K19; K1CS; KRT19
UniProt ID: (Human) P08727
Entrez Gene ID: (Human) 3880
