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Invitrogen
DARPP-32 Polyclonal Antibody
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DARPP-32 Antibody (PA5-85788) in ICC/IF
DARPP-32 Polyclonal Antibody detects DARPP-32 protein at cell body by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: DARPP-32 protein stained by DARPP-32 Polyclonal Antibody (Product # PA5-85788) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta-3 Tubulin Polyclonal Antibody [GT11710] diluted at 1:500. Fluoroshield with DAPI .
产品信息
PA5-85788
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat
已发表种属
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the C-terminus region of mouse DARPP-32. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.27 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol, 1% BSA
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (86%).
Positive Control: mouse brain, rat brain.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
DARPP-32 is a dopamine (DA) and AMP-regugated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons. The protein inhibits Protein Phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regugation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine- and cAMP-regulated phosphoprotein DARPP-32; FLJ20940; IPPD; neuronal phosphoprotein DARPP-32; OTTHUMP00000164275; OTTHUMP00000164276; PPR1B; protein pho; Protein phosphatase 1 regulatory subunit 1B
基因别名: AU040756; DARPP-32; Darpp32; Ppp1r1b
UniProt ID: (Rat) Q6J4I0, (Mouse) Q60829
Entrez Gene ID: (Rat) 360616, (Mouse) 19049
