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Identifyn
BRCA1 Recombinant Rabbit Monoclonal Antibody (BLR023N), Alexa Fluor™ 680, Identifyn™
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FIGURE: 1 / 7
BRCA1 Antibody (DC-000047) in ICC/IF
Identifyn Detailed protocols can be found below under Documents & Downloads. Confocal Microscopy: HeLa cells were grown on acid-etched 1.5H cover glass to ~50% confluency. DNA damage was induced via treatment with 2 µM Etoposide–spiked complete growth media for 24 hours, followed by -20°C methanol fixation and blocked with Thermo Fisher Fish Serum Blocker (Product # 37527X3). The primary antibody, Rabbit anti-HumanBRCA1 Recombinant Monoclonal - Identifyn SRM Alexa Fluor 680, IgG, was incubated for 1 hour at room temperature at a 1:100 dilu... View More
Product Details
DC-000047
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
ExpiCHO
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR023N
Immunogen
Synthetic peptides within human BRCA1 map to a region between residues 700 and 750 of human breast cancer. The exactimmunogen used to generate this antibody is proprietary information; a database link to Uniprot and Gene ID is provided..
Conjugate
Alexa Fluor™ 680
Alexa Fluor™ 680
Alexa Fluor™ 680
Excitation/Emission Max
681/704 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 10% glycerol
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan and STORM Microscopy.
Recommended dilution for traditional microscopy:
Widefield - 1:250-1:1,000
Widefield SIM - 1:250-1:1,000
Confocal - 1:250-1:1,000
Recommended dilution for Super Resolution microscopy:
SIM (Structured Illumination Microscopy) 1:100-1:1,000
Single Molecule STORM (Stochastic Optical Reconstruction Microscopy) - 1:100-1:1,000
Detailed protocols can be found below under Documents & Downloads.
Target Information
BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor in combination with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. The BRCA1 protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: brca 1; brca-1; BRCA1/BRCA2-containing complex, subunit 1; breast and ovarian cancer susceptibility protein 1; breast cancer 1 early onset; breast cancer 1, early onset; Breast cancer type 1 susceptibility protein; breast cancer type 1 susceptibility protein isoform p.23; breast cancer type 1 susceptibility protein, isoform p.10; breast cancer type 1 susceptibility protein, isoform p.11; breast cancer type 1 susceptibility protein, isoform p.12; breast cancer type 1 susceptibility protein, isoform p.13; breast cancer type 1 susceptibility protein, isoform p.14; breast cancer type 1 susceptibility protein, isoform p.15; breast cancer type 1 susceptibility protein, isoform p.16; breast cancer type 1 susceptibility protein, isoform p.17; breast cancer type 1 susceptibility protein, isoform p.18; breast cancer type 1 susceptibility protein, isoform p.19; breast cancer type 1 susceptibility protein, isoform p.20; breast cancer type 1 susceptibility protein, isoform p.21; breast cancer type 1 susceptibility protein, isoform p.22; breast cancer type 1 susceptibility protein, isoform p.24; breast cancer type 1 susceptibility protein, isoform p.25; breast cancer type 1 susceptibility protein, isoform p.26; breast cancer type 1 susceptibility protein, isoform p.27; breast cancer type 1 susceptibility protein, isoform p.28; breast cancer type 1 susceptibility protein, isoform p.29; breast cancer type 1 susceptibility protein, isoform p.30; breast cancer type 1 susceptibility protein, isoform p.31; breast cancer type 1 susceptibility protein, isoform p.32; breast cancer type 1 susceptibility protein, isoform p.33; breast cancer type 1 susceptibility protein, isoform p.34; breast cancer type 1 susceptibility protein, isoform p.35; breast cancer type 1 susceptibility protein, isoform p.36; breast cancer type 1 susceptibility protein, isoform p.37; breast cancer type 1 susceptibility protein, isoform p.38; breast cancer type 1 susceptibility protein, isoform p.39; breast cancer type 1 susceptibility protein, isoform p.40; breast cancer type 1 susceptibility protein, isoform p.41; breast cancer type 1 susceptibility protein, isoform p.42; breast cancer type 1 susceptibility protein, isoform p.6; breast cancer type 1 susceptibility protein, isoform p.7; breast cancer type 1 susceptibility protein, isoform p.8; breast cancer type 1 susceptibility protein, isoform p.9; BROVCA12; Delta772-3095; early onset breast cancer 1; Fanconi anemia, complementation group S; influences susceptibility to breast and ovarian cancer; OTTHUMP00000212147; OTTHUMP00000212148; OTTHUMP00000212149; OTTHUMP00000212150; OTTHUMP00000212151; OTTHUMP00000212155; protein phosphatase 1, regulatory subunit 53; RING finger protein 53; RING-type E3 ubiquitin transferase BRCA1; unnamed protein product
Gene Aliases: BRCA1; BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53
UniProt ID: (Human) P38398
Entrez Gene ID: (Human) 672
transcription regulatory region sequence-specific DNA binding
p53 binding
DNA binding
damaged DNA binding
transcription coactivator activity
RNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
tubulin binding
transferase activity
enzyme binding
ubiquitin protein ligase binding
identical protein binding
metal ion binding
ubiquitin protein ligase activity
ubiquitinated histone binding
RNA polymerase binding
histone H2AK127 ubiquitin ligase activity
histone H2AK129 ubiquitin ligase activity
double-strand break repair via homologous recombination
DNA repair
regulation of DNA repair
postreplication repair
double-strand break repair
DNA recombination
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
lipid metabolic process
fatty acid metabolic process
fatty acid biosynthetic process
cellular response to DNA damage stimulus
chromosome segregation
mitotic G2 DNA damage checkpoint
centrosome cycle
dosage compensation
intrinsic apoptotic signaling pathway in response to DNA damage
response to ionizing radiation
positive regulation of vascular endothelial growth factor production
positive regulation of gene expression
protein ubiquitination
negative regulation of cell growth
negative regulation of intracellular estrogen receptor signaling pathway
reciprocal DNA recombination
chordate embryonic development
hypermethylation of CpG island
mitotic G2/M transition checkpoint
negative regulation of fatty acid biosynthetic process
positive regulation of DNA repair
positive regulation of angiogenesis
negative regulation of cell cycle
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of centriole replication
regulation of cell cycle
protein autoubiquitination
random inactivation of X chromosome
cellular response to tumor necrosis factor
cellular response to ionizing radiation
cellular response to indole-3-methanol
protein K6-linked ubiquitination
DNA strand resection involved in replication fork processing
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
regulation of DNA damage checkpoint
negative regulation of reactive oxygen species metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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