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Identifyn
ATRIP Monoclonal Antibody (4H6H4/G3), Alexa Fluor™ 555, Identifyn™
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FIGURE: 1 / 7
ATRIP Antibody (DC-000071) in ICC/IF
Identifyn Detailed protocols can be found below under Documents & Downloads. Widefield SIM: HeLa cells were grown on acid-etched 1.5H cover glass to ~50% confluency, then fixed in -20°C methanol and blocked with Thermo Fisher Fish Serum Blocker (Product # 37527X3). The primary antibody, Mouse anti-Human ATRIP Monoclonal - Identifyn SRM Alexa Fluor 555, IgG2b - (4H6H4/G3), was incubated for 1 hour at room temperature at a dilution of 1:250. Cells were washed 3X in PBS. The second primary antibody, Actin Recombinant Rabbit Monoclonal Antibod... View More
Product Details
DC-000071
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
4H6H4/G3
Immunogen
Immunogen against recombinant full-length protein, Uniprot ID Q8WXE1, Gene ID 84126.
Conjugate
Alexa Fluor™ 555
Alexa Fluor™ 555
Alexa Fluor™ 555
Excitation/Emission Max
553/568 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 10% glycerol
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan and STORM Microscopy.
Recommended dilution for traditional microscopy:
Widefield - 1:250-1:1,000
Widefield SIM - 1:250-1:1,000
Confocal - 1:250-1:1,000
Recommended dilution for Super Resolution microscopy:
SIM (Structured Illumination Microscopy) 1:100-1:1,000
Single Molecule STORM (Stochastic Optical Reconstruction Microscopy) - 1:100-1:1,000
Detailed protocols can be found below under Documents & Downloads.
Target Information
ATRIP is an 86 kDa protein that is phosphorylated by ATR, regulates ATR expression, and is an essential component of the DNA damage checkpoint pathway. It localizes to the intranuclear foci after DNA damage or inhibition of replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATM and Rad3-related-interacting protein; ATR IP; ATR-interacting protein; DNase III; DRN3; FLJ12343; unnamed protein product
Gene Aliases: AGS1; ATRIP
UniProt ID: (Human) Q8WXE1
Entrez Gene ID: (Human) 84126
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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