Identifyn

gamma-H2AX SRM Recombinant Rabbit Monoclonal Antibody (BLR014M), Alexa Fluor™ 594, Identifyn™

Name: gamma-H2AX SRM Recombinant Rabbit Monoclonal Antibody (BLR014M), Alexa Fluor\u2122 594, Identifyn\u2122
Brand: Identifyn
SKU: DC000019

查看其他17个gamma-H2AX抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (8)

gamma-H2AX SRM Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

gamma-H2AX SRM Antibody (DC000019) in ICC/IF

IdentifynTM detailed protocols can be found below under Documents & Downloads. Western Blot: HEK 293 cells were damaged with 200 µM etoposide for 24 hours. Cells were then homogenized using an ultrasonication probe and RIPA lysis buffer and then centrifuged to pellet down the cell debris. The lysate supernatant (100 µg) was mixed with NuPAGE LDS Sample Buffer (4X), heated at 80 °C for 10 min, and subjected ... View More

Product Image Gallery

Immunogen image

Immunogen Image gamma-H2AX SRM Recombinant Rabbit Monoclonal Antibody (BLR014M), Alexa Fluor™ 594, Identifyn™

产品信息

DC000019

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫细胞化学 (ICC/IF)

1:200-1:1,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

ExpiCHO

分类

Recombinant Monoclonal

类型

Antibody

克隆号

BLR014M

抗原

Proprietary peptide specific to serine 139 phosphorylation.

查看免疫原

偶联物

Alexa Fluor™ 594 Alexa Fluor™ 594 Alexa Fluor™ 594

激发/发射光谱

590/618 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 10% glycerol

内含物

0.02% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan and STORM Microscopy.

Recommended dilution for traditional microscopy:

Widefield - 1:1,000

Widefield SIM - 1:1,000

Confocal - 1:1,000

Recommended dilution for Super Resolution microscopy:

SIM (Structured Illumination Microscopy) - 1:1,000

Single Molecule STORM (Stochastic Optical Reconstruction Microscopy) - 1:200

Detailed protocols can be found below under Methods & Protocols.

靶标信息

H2AX is a variant of the H2A protein family and is a component of the histone octomer in nucleosomes. H2AX is phosphorylated by kinases such as ataxia telangiectasia mutated (ATM) and ATM-Rad3-related (ATR) in the PI3K pathway. Gamma-H2AX is the first step in recruiting and localizing DNA repair proteins. DSBs can be induced by mechanisms such as ionizing radiation or cytotoxic agents and subsequently, gamma-H2AX foci quickly form. These foci represent the DSBs in a 1:1 manner and can be used as a biomarker for damage. The visualization of gamma-H2AX by can allow the assessment of DNA damage, related DNA damage proteins and DNA repair. Gamma-H2AX has applications in the detection of genomic damage caused by cytotoxic chemical agents and environmental and physical damage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。