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Agrisera
DCL2/Dicer-like protein 2 Polyclonal Antibody
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DCL2/Dicer-like protein 2 Antibody (AS15-3100) in WB
50 µg of total protein from Arabidopsis thaliana whole vegetative rosette, DCL 2 overexpression line (a), wild type Col-0 (b), dcl 2-1 intron insertion (c), extracted with extraction buffer (50 m M Tris p H 7.5; 150 m M Na Cl; 1 m M EDTA; 10% v/v Glycerin; 1 m M DTT, 1 x Complete Protease Inhibitor Cocktail, Roche) and denatured with LaemmLi buffer at 95°C/5 min. were separated on 7.5% SDS-PAGE and blotted ... View More
产品信息
AS15-3100
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana DCL2 sequence.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
DCL2 encodes a predominantly nuclear, Dicer-like RNase III endoribonuclease that functions in RNA interference pathways by recognizing double-stranded RNA substrates and processing them into small interfering RNAs, with a prominent role in antiviral silencing that becomes especially evident when the related DCL4 pathway is compromised; it can also contribute to endogenous small-RNA production, including trans-acting siRNA biogenesis, and has been implicated in maintaining aspects of DNA methylation-linked silencing. The DCL2 protein is a large, multidomain enzyme with an N-terminal DExD/H-box helicase module (ATP-dependent RNA remodeling), followed by a DUF283 region, a PAZ domain that helps engage RNA termini, two tandem RNase III catalytic domains that form the dimeric active center for staggered dsRNA cleavage, and C-terminal double-stranded RNA-binding domain(s) that stabilize substrate interactions; multiple alternatively spliced transcript variants are reported, consistent with potential isoform-level modulation of activity and/or regulation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT3G03300; ATDCL2; DICER-LIKE 2; T17B22.28
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