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DCL3/Dicer-like protein 3 Polyclonal Antibody
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DCL3/Dicer-like protein 3 Antibody (AS12-2103) in WB
50 µg of total protein Arabidopsis thaliana total cell extract which came from the ground, frozen powder and has been directly transferred to 2 x LaemmLi sample buffer was separated on 15% SDS-PAGE and blotted over night at 64 m A to nitrocellulose (wet transfer). Blots were blocked with Roti-block over night at 4°C agitation. Blot was incubated in the primary antibody at a dilution of 1:2,000(1)and 1:500(2... View More
产品信息
AS12-2103
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DCL3 sequence.
偶联物
形式
Lyophilized
规格
200 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 350 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
DCL3 is a nuclear, protein-coding gene in Arabidopsis thaliana that produces a large multidomain RNase III family endoribonuclease of the Dicer-like class. The DCL3 protein is organized in the canonical Dicer architecture, with an N-terminal DExD/H-box helicase module followed by a helicase C region, a DUF283 segment implicated in macromolecular interactions, a PAZ domain that recognizes the termini of double-stranded RNA substrates, and a C-terminal catalytic core containing tandem RNase III domains plus double-stranded RNA-binding domain(s) required for precise duplex cleavage. Functionally, DCL3 is essential for endogenous RDR2-dependent small interfering RNA biogenesis and is specialized for generating predominantly 24-nucleotide small interfering RNAs from double-stranded RNA precursors produced in the RNA-directed DNA methylation pathway; these 24-nucleotide products guide transcriptional silencing and epigenetic regulation of repetitive and heterochromatic loci through downstream effector complexes, and DCL3 activity is also implicated in methylation-mediated defense against DNA viruses via the same silencing machinery.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT3G43920; ATDCL3; dicer-like 3
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