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Agrisera
DCL5/Zea mays Dicer-like 102 Polyclonal Antibody
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产品信息
AS22-4722
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Zea mays DCL5 protein sequence.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
靶标信息
DCL5 encodes a cytoplasmic/nuclear RNA-silencing endoribonuclease that localizes to reproductive tissues, with particularly strong functional relevance in developing monocot anthers, where it supports the small-RNA program of the tapetum during meiosis. The gene produces a multi-domain Dicer-class protein with the canonical architecture used for double-stranded RNA processing: an N-terminal DExD/H-box helicase region followed by a DUF283-like segment, a central PAZ RNA-end-binding domain that helps define cleavage registers, tandem RNase III catalytic domains that execute staggered cuts in duplex RNA, and a C-terminal double-stranded RNA-binding domain. Biologically, DCL5 is required for the biogenesis of abundant 24-nt phased small interfering RNAs (24-nt phasiRNAs) from precursor transcripts in monocots; loss of DCL5 markedly depletes these 24-nt phasiRNAs, disrupts normal tapetal development and anther growth, and can cause temperature-sensitive male sterility, indicating that DCL5-dependent phasiRNA production contributes to robust male fertility under agronomically relevant growth conditions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DCL5; dicer-like 5
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