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Agrisera
DCP5/Protein decapping 5 Polyclonal Antibody
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DCP5/Protein decapping 5 Antibody (AS23-4944) in WB
Samples: 1 - HA-DCP 1 (transient in N. Benthamiana) 2 - HA-DCP 2 (transient in N. Benthamiana) 3 - HA-DCP 5 (transient in N. Benthamiana) Total protein extracted freshly from 2 leaf discs (Ø6 mm) of 4-week-old Nicotiana benthamiana or Arabidopsis thaliana leaves in 150 µL extraction buffer (100 m M Tris p H=7.5,1 m M EDTA, 3% SDS) + 50 µL 4 X LaemmLi buffer (Bio-Rad) and denatured at 95°C for 10 min. Sample... View More
产品信息
AS23-4944
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana DCP5.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Nicotiana benthamiana (transiently expressed)
靶标信息
DCP5 encodes a cytoplasmic RNA granule protein that concentrates in discrete processing bodies (P-bodies), where it colocalizes and physically associates with core mRNA decapping factors including DCP1 and the catalytic decapping enzyme DCP2. The DCP5 protein is built around an N-terminal Lsm domain followed by a Ser/Thr-rich low-complexity region and a C-terminal segment containing two RGG-rich regions separated by an FDF motif, a modular architecture consistent with RNA/protein interactions and ribonucleoprotein condensate assembly. Functionally, genetic and biochemical data support DCP5 as an mRNA decapping activator and P-body nucleator required for efficient decapping in vivo (without directly stimulating DCP2 catalysis in vitro), and as a translational repressor that helps keep selected mRNAs (including seed storage protein transcripts during germination) translationally silent and routed toward decay; more recently, DCP5 has also been shown to participate in transcriptional control of FLOWERING LOCUS C in a mechanism involving phase-separation behavior mediated by prion-like features.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT1G26110; F14G11.8; F14G11_8; F28B23.21
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