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Invitrogen
DDB1 Polyclonal Antibody
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图: 1 / 11
DDB1 Antibody (PA5-79142) in ICC/IF
Immunocytochemistry analysis of DDB1 using anti-DDB1 antibody (Product # PA5-79142). DDB1 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-DDB1 antibody (Product # PA5-79142) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize usin... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-79142
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human DDB1 recombinant protein (Position: S1011-H1140).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human HepG2 whole cell, human HEK293 whole cell, human K562 whole cell, human Raji whole cell, human CACO-2 whole cell, human THP-1 whole cell, rat brain tissue, rat kidney tissue, rat C6 whole cell, mouse brain tissue, mouse kidney tissue, mouse testis tissue. IHC: Mouse Liver tissue, Rat Liver tissue, Human Intestinal Cancer tissue IHC-F: human placenta tissue. ICC/IF: A431 cell, A549 Cell, PC-3 Cell, SMMC-7721 Cell. Flow: 293T cell.
靶标信息
The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: damage specific DNA binding protein 1; Damage-specific DNA-binding protein 1; damage-specific DNA-binding protein, DNA repair; damaged-DNA recognition protein 1; DDB p127 subunit; DDBa; DNA damage-binding protein 1; DNA damage-binding protein a; DNA repair protein; HBV X-associated protein 1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; XAP-1; xeroderma pigmento; Xeroderma pigmentosum group E-complementing protein; XPCe; XPE-BF; XPE-binding factor
基因别名: 127kDa; AA408517; DDB1; p127-Ddb1; XAP1
UniProt ID: (Mouse) Q3U1J4
Entrez Gene ID: (Mouse) 13194, (Rat) 64470
DNA repair
nucleotide-excision repair
cellular response to DNA damage stimulus
Wnt signaling pathway
histone H2A monoubiquitination
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of viral genome replication
positive regulation by virus of viral protein levels in host cell
interaction with symbiont
UV-damage excision repair
regulation of mitotic cell cycle phase transition
positive regulation of viral release from host cell
protein ubiquitination
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