Invitrogen

DDR2 Polyclonal Antibody

Name: DDR2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-27752

高级验证

查看其他13个DDR2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (7)
高级验证 (1)

DDR2 Antibody in Immunocytochemistry (ICC/IF)

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DDR2 Antibody (PA5-27752) in ICC/IF

Immunofluorescence analysis of DDR2 was performed using 70% confluent log phase HT-1080 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with DDR2 Polyclonal Antibody (Product # PA5-27752) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32... View More

DDR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DDR2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-DDR2 Polyclonal Antibody (Product # PA5-27752), shows increased expression of DDR2 in HT-1080 as compared to MCF7 which is known to be a low expressing cell line (DOI: 10.18632/oncotarget.8795). {RE}

产品信息

PA5-27752

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:200

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the Intracellular domain of human DDR2. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.43 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2545228

产品详细信息

Recommended positive controls: Raji, mouse brain.

Predicted reactivity: Mouse (97%), Rat (97%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

DDR2 is a member of a subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. DDR1 and DDR2 have been shown to be potently inhibited by Gleevec. DDR2 required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD167 antigen-like family member B; CD167b; CD_antigen: CD167b; cell migration-inducing protein 20; Discoidin domain receptor 2; discoidin domain receptor family, member 2; Discoidin domain-containing receptor 2; Discoidin domain-containing receptor tyrosine kinase 2; hydroxyaryl-protein; hydroxyaryl-protein kinase; migration-inducing gene 16 protein; neurotrophic tyrosine kinase receptor related 3; Neurotrophic tyrosine kinase, receptor-related 3; Receptor protein-tyrosine kinase TKT; RP11-572K18.1; Tyrosine-protein kinase TYRO10; tyrosylprotein kinase

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基因别名: AW495251; DDR2; MIG20a; NTRKR3; TKT; TYRO10

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UniProt ID: (Human) Q16832, (Mouse) Q62371

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Entrez Gene ID: (Human) 4921, (Mouse) 18214

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