Invitrogen

DDX1 Monoclonal Antibody (2D4)

Name: DDX1 Monoclonal Antibody (2D4)
Brand: Invitrogen
SKU: MA5-49263

查看其他13个DDX1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (7)

DDX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DDX1 Antibody (MA5-49263) in IHC (P)

Immunohistochemistry analysis of DDX1 in paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with DDX1 Monoclonal antibody (Product # MA5-49263) using a dilution of 2 μg/mL overnight at 4°C. Biotinylated goat anti-m... View More

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Immunogen Image DDX1 Monoclonal Antibody (2D4)

产品信息

MA5-49263

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.25 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

2D4

抗原

E.coli-derived human DDX1 recombinant protein (Position: K562-F740).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_3074921

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human K562 whole cell, human CACO-2 whole cell, human U20S whole cell, human HEK293 whole cell, human U87 whole cell, human HELA whole cell, human A549 whole cell, rat kidney tissue, mouse kidney tissue. IHC: human gastric cancer tissue, human pancreatic cancer tissue, rat brain tissue, human esophageal squamous carcinoma tissue. Flow: MCF-7 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

DDX1 (ATP-dependent RNA helicase DDX1) acts as an ATP-dependent RNA helicase. It is able to unwind both RNA-RNA and RNA-DNA duplexes. DDX1 possesses 5' single-stranded RNA overhand nuclease activity and ATPase activity on various RNA, but not DNA polynucleotides. DDX1, together with RELA, acts as a coactivator to enhance NF-kappa-b-mediated transcriptional activation. It also acts as a positive transcriptional regulator of cyclin CCND2 expression. It binds to the cyclin CCND2 promoter region. DDX1 is a component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double stranded RNA and plays a role in activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: ATP-dependent RNA helicase DDX1; DBP-RB; DEAD (Asp-Glu-Ala-Asp) box helicase 1; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD box polypeptide 1; DEAD box protein 1; DEAD box protein retinoblastoma; DEAD box-1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1

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基因别名: AA409185; DBP-RB; DDX1; UKVH5d

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UniProt ID: (Human) Q92499, (Rat) Q641Y8, (Mouse) Q91VR5

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Entrez Gene ID: (Human) 1653, (Rat) 84474, (Mouse) 104721

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