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Invitrogen
DFNA5 Monoclonal Antibody (A1H3)
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图: 1 / 7
DFNA5 Antibody (MA5-36092) in ICC/IF
Immunocytochemical analysis of DFNA5 in SiHa cells using a DFNA5 monoclonal antibody (Product #MA5-36092). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI.
产品信息
MA5-36092
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
A1H3
抗原
Recombinant protein within Human DFNA5 aa 34-214.
偶联物
形式
Liquid
浓度
5.351 mg/mL
规格
535.1 µg
纯化类型
Protein G
保存液
PBS, pH 7.4, with 0.2% BSA, 50% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
GSDME (Gasdermin E) is a member of the gasdermin family of pore-forming proteins that regulate programmed cell death and inflammatory signaling. Encoded by the GSDME gene, the full-length protein contains an N-terminal pore-forming domain and a C-terminal autoinhibitory domain. Under apoptotic conditions, activation of caspase-3 leads to proteolytic cleavage of GSDME, releasing the N-terminal fragment, which oligomerizes and inserts into the plasma membrane to form pores. This process can convert non-lytic apoptosis into a lytic, inflammatory form of cell death resembling pyroptosis, resulting in membrane rupture and release of intracellular contents. GSDME has been implicated in cancer biology, chemotherapy response, and tissue injury, and was originally identified in association with autosomal dominant hearing loss (historically referred to as DFNA5). Detection of cleaved GSDME is commonly used as a marker of caspase-3-mediated pore formation and inflammatory cell death.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DFNA5; DFNA5, deafness associated tumor suppressor; expression inversely correlated with estrogen receptor expression in breast cancer; Gasdermin E; Gasdermin-E; ICERE-1; Inversely correlated with estrogen receptor expression 1; Non-syndromic hearing impairment protein 5; nonsyndromic hearing impairment protein; unnamed protein product
基因别名: DFNA5; GSDME; ICERE-1; ICERE1
UniProt ID: (Human) O60443
Entrez Gene ID: (Human) 1687
positive regulation of immune response to tumor cell
sensory perception of sound
negative regulation of cell proliferation
programmed cell death
auditory receptor cell differentiation
positive regulation of MAPK cascade
transmembrane transport
pyroptosis
cellular response to tumor necrosis factor
cellular response to virus
granzyme-mediated programmed cell death signaling pathway
pyroptotic cell death
positive regulation of intrinsic apoptotic signaling pathway
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