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Agrisera
DGAT2A/Acyl-CoA: Diacylglycerol acyltransferase Polyclonal Antibody
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DGAT2A/Acyl-CoA: Diacylglycerol acyltransferase Antibody (AS12-1874) in WB
Total proteins (containing 30 µg ) from Chlamydomonas reinhardtii cells grown for the indicated times in N-deprived medium extracted with lysis buffer (50 m M Tris-HCl, p H 6.8, containing 2% SDS and 10 m M EDTA and a protease inhibitor cocktail) were separated on 12% SDS-PAGE and transferred onto a nitrocellose blot over night at 4°C. Blots were blocked with blocking buffer ( 5% (w/v) non-fat dry milk powd... View More
产品信息
AS12-1874
产品规格
种属反应
Algae
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant CrDGAT2A, overexpressed in E.coli, missing transmembrane domains.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 100 µL of sterile water.
Specific Species Reactivity: Chlamydomonas reinhardtii
靶标信息
DGAT2A encodes an acyl-CoA:diacylglycerol acyltransferase-family enzyme that localizes predominantly to the endoplasmic reticulum (ER) and can redistribute to the surface of cytosolic lipid droplets during fatty-acid loading, consistent with a role at ER-lipid droplet interfaces that support neutral-lipid deposition. The protein is an integral membrane acyltransferase predicted to contain two transmembrane helices, with a short luminal loop connecting them, while the catalytically important regions reside in a larger membrane-associated cytosolic domain; DGAT2-family proteins can also assemble into higher-order complexes and physically associate with upstream acyltransferases that generate diacylglycerol, helping couple substrate supply to triacylglycerol production. Functionally, DGAT2A catalyzes the terminal, committed step of triacylglycerol synthesis by transferring a fatty acyl group from long-chain acyl-CoA to diacylglycerol, thereby promoting triacylglycerol accumulation and lipid droplet growth; this activity supports cellular energy storage and buffering of excess fatty acids, and is implicated in tissue lipid handling in metabolic contexts where neutral-lipid synthesis and droplet expansion are dynamically regulated.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DGAT2A; UrDGAT2A
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