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Agrisera
DHAR1/Dehydroascorbate Reductase 1 Polyclonal Antibody
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DHAR1/Dehydroascorbate Reductase 1 Antibody (AS11-1746) in WB
1 cm 2 of a leaf from Arabidopsis thaliana Col-0 (1) and or t-DNA insertion lines dhar 1-1 (2), dhar 1-2 (3), dhar 1-3 (4), dhar 2-1 (5), dhar 2-2 (6), dhar 1-3 EOS-DHAR 1 (7), was extracted using 200 µL Lyse& Load-Buffer (Grefen et al. 2009). 10 µL were separated on a 15% SDS-PAGE and blotted 1 h to PVDF (using Bjerrum Buffer in a semidry blot). Blots were blocked with 5% Milk in 1 x TBS-Tween 20 (1%) for ... View More
产品信息
AS11-1746
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from known DHAR1 sequence of Arabidopsis thaliana.
偶联物
形式
Lyophilized
规格
200 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 200 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Kandelia candel, Solanum lycopersicum
靶标信息
DHAR1 encodes a dehydroascorbate reductase that functions as a soluble, cytosolic enzyme in Arabidopsis thaliana, contributing to cellular redox homeostasis by supporting the ascorbate-glutathione cycle. The DHAR1 protein is a small (~213 aa) glutathione-dependent oxidoreductase with a thioredoxin-like fold and glutathione S-transferase-like N- and C-terminal regions typical of plant DHARs, consistent with its role in binding glutathione and reducing dehydroascorbate back to ascorbate. Genetically, loss of DHAR1 reduces total DHAR activity in leaves and compromises maintenance of the ascorbate redox state during photooxidative stress, leading to heightened stress symptoms compared with wild type, indicating DHAR1 is a key enzymatic component for ascorbate recycling and protection against reactive oxygen species in planta.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ATDHAR1; dehydroascorbate reductase; DEHYDROASCORBATE REDUCTASE 5; DHAR5; F14P1.9; F14P1_9
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