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Invitrogen
DHCR7 Polyclonal Antibody
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DHCR7 Antibody (PA5-115116) in ICC/IF
Immunocytochemistry analysis of DHCR7 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115116) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-115116
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human DHCR7(Accession Q9UBM7), corresponding to amino acid residues M1-P51.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total DHCR7.
靶标信息
Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 7-DHC reductase; cholesterol-5,6-epoxide hydrolase subunit DHCR7; D7SR; delta-7-dehydrocholesterol reductase; delta7-sterol reductase; Dhcr7; putative sterol reductase SR-2; sterol Delta(7)-reductase; sterol delta-7-reductase; sterol reductase; sterol reductase SR-2; unnamed protein product
基因别名: SLOS
UniProt ID: (Human) Q9UBM7, (Rat) Q9Z2Z8, (Mouse) O88455
Entrez Gene ID: (Human) 1717, (Rat) 64191, (Mouse) 13360
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