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Rockland
DIA-2 Polyclonal Antibody
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DIA-2 Antibody (600-401-851) in WB
Western blot using Rocklands Affinity Purified anti-DIA-2 antibody shows detection of a 132-kDa band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
产品信息
600-401-851
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues at the C-Terminus of Human DIA-2.
偶联物
形式
Liquid
浓度
1.4 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human DIA-2. A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. However, cross reactivity is expected with mouse DIA-2 based on a 93% homology to the immunogen sequence. Cross reactivity with DIA-2 homologues from other sources has not been determined.
靶标信息
The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: diaphanous homolog 2; diaphanous-related formin 2; Diaphanous-related formin-2; diaphorase-2; DRF2; FLJ11167; mDia3; Protein diaphanous homolog 2; RP11-212B22.1
基因别名: DIA; DIA2; DIAPH2; DRF2; POF; POF2
UniProt ID: (Human) O60879
Entrez Gene ID: (Human) 1730
