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Invitrogen
DIO3 Polyclonal Antibody
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FIGURE: 1 / 5
DIO3 Antibody (PA5-26537) in IHC (P)
Immunohistochemistry analysis of DIO3 in formalin-fixed and paraffin-embedded human hepatocarcinoma. Samples were incubated with DIO3 polyclonal antibody (Product # PA5-26537) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Details
PA5-26537
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 250-278 amino acids from the C-terminal region of human DIO3
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
DIO3 belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 5DIII; deiodinase, iodothyronine, type 3; DIOIII; iodothyronine deiodinase, placental type; Thyroxine 5-deiodinase; thyroxine deiodinase; thyroxine deiodinase type III (selenoprotein); Type 3 DI; type 3 iodothyronine selenodeiodinase; Type III iodothyronine deiodinase; type-III 5' deiodinase; type-III 5'-deiodinase
Gene Aliases: 5DIII; D3; DIO3; DIOIII; ITDI3; TXDI3
UniProt ID: (Human) P55073, (Rat) P49897
Entrez Gene ID: (Human) 1735, (Rat) 29475
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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