Invitrogen

DLL1 Polyclonal Antibody

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1 篇参考文献

查看其他24个DLL1抗体

问题与答案

问题与答案

抗体检测数据 (3)
已发表图片 (1)

DLL1 Antibody in Western Blot (WB)

DLL1 Antibody in Western Blot (WB)

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DLL1 Antibody (PA5-42902) in WB

Western blot was performed using Anti-DLL1 Polyclonal Antibody (Product # PA5-42902) and ~78 kDa band corresponding to DLL1 was observed across all the cell lines and tissues tested. Membrane extracts (30 µg lysate) of IMR-32 (Lane 1), SH-SY5Y (Lane 2), PANC-1 (Lane 3), MCF7 (Lane 4), THP-1 (Lane 5), Mouse Spleen (Lane 6), Rat Spleen (Lane 7), Rat Heart (Lane 8) and Mouse Heart (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane... View More

DLL1 Antibody in Western Blot (WB)

DLL1 Antibody in Western Blot (WB)

DLL1 Antibody in Western Blot (WB)

DLL1 Antibody in Western Blot (WB)

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DLL1 Antibody in Western Blot (WB) — Western blot was performed using Anti-DLL1 Polyclonal Antibody (Product # PA5-42902) and ~78 kDa band corresponding to DLL1 was observed across all the cell lines and tissues tested. Membrane extracts (30 µg lysate) of IMR-32 (Lane 1), SH-SY5Y (Lane 2), PANC-1 (Lane 3), MCF7 (Lane 4), THP-1 (Lane 5), Mouse Spleen (Lane 6), Rat Spleen (Lane 7), Rat Heart (Lane 8) and Mouse Heart (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

DLL1 Antibody in Western Blot (WB) — Western blot analysis of Hela cell lysate using an anti-DLL1 polyclonal antibody (Product # PA5-42902). Lane 1) Primary Antibody; Lane 2) Primary Antibody + Blocking Peptide.

DLL1 Antibody in Western Blot (WB) — Western blot analysis of human HeLa cell lysate using an anti-DLL1 polyclonal antibody (Product # PA5-42902).

DLL1 Antibody in Western Blot (WB) — Fig. 4 DLL1 overexpression improves the myogenic propensity of mesodermal progenitors. A Schematic overview of the doxycycline (dox)-inducible DLL1 construct flipped into an embryonic stem cell (ESC) master line. B Quantitative PCR for DLL1 after exposure of the generated ESC line to dox for two or four days. C Western blot for DLL1 and NOTCH1 after exposure of the generated ESC line to dox for four days. Tubulin (TUB) was used as a housekeeping protein. Quantification graph is on the right. D Immunofluorescence analysis of lamin A/C-positive mesodermal progenitors, with or without dox, (green) in co-culture with C2C12 myoblasts ( n = 3). Scale bars: 100 um. * P < 0.05.

Immunogen image

DLL1 Polyclonal Antibody

Immunogen Image DLL1 Polyclonal Antibody

产品信息

PA5-42902

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2-1 µg/mL

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免疫组化 (IHC)

5 µg/mL

-

产品规格

种属反应

Human, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide directed towards the N-terminal of human DLL1 (aa 2-51).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Peptide sequence: GSRCALALAV LSALLCQVWS SGVFELKLQE FVNKKGLLGN RNCCRGGAGP

Sequence homology: Cow: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 93%; Rat: 100%

靶标信息

Delta-like protein 1 (DLL1) is a transmembrane protein characterized by epidermal growth factor (EGF)-like repeats. It is a ligand for Notch receptor. It blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Delta-like protein 1; Delta1; Drosophila Delta homolog 1; H-Delta-1; OTTHUMP00000017690

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基因别名: Delta; DELTA1; DL1; DLL1; UNQ146/PRO172

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UniProt ID: (Human) O00548, (Rat) P97677

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Entrez Gene ID: (Human) 28514, (Rat) 84010

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Notch binding calcium ion binding protein binding Tat protein binding scaffold protein binding

cell fate determination somitogenesis somite specification heart looping Notch signaling pathway cell-cell signaling determination of left/right symmetry compartment pattern specification nervous system development auditory receptor cell fate commitment negative regulation of interleukin-10 production negative regulation of cell differentiation negative regulation of auditory receptor cell differentiation negative regulation of myeloid cell differentiation negative regulation of neuron differentiation positive regulation of Notch signaling pathway positive regulation of transcription from RNA polymerase II promoter inner ear development regulation of neurogenesis left/right axis specification proximal tubule development loop of Henle development neuronal stem cell population maintenance marginal zone B cell differentiation type B pancreatic cell development Notch receptor processing regulation of blood pressure positive regulation of cell proliferation negative regulation of cell proliferation proximal/distal pattern formation astrocyte development regulation of somitogenesis spinal cord development cerebellar molecular layer formation cerebellar Purkinje cell layer structural organization hemopoiesis cell differentiation regulation of cell adhesion negative regulation of epithelial cell differentiation negative regulation of glial cell apoptotic process organ growth regulation of growth negative regulation of epidermal cell differentiation negative regulation of myoblast differentiation positive regulation of endocytosis lateral inhibition skeletal muscle tissue growth regulation of skeletal muscle tissue growth positive regulation of skeletal muscle tissue growth neuron fate specification regulation of cell division retina development in camera-type eye retina morphogenesis in camera-type eye Notch signaling pathway involved in arterial endothelial cell fate commitment nephron development clathrin-mediated endocytosis endothelial tip cell fate specification skin epidermis development regulation of vascular endothelial growth factor signaling pathway positive regulation of sprouting angiogenesis regulation of energy homeostasis