Invitrogen

DNMT1 Monoclonal Antibody (60B1220.1), APC

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说明书
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说明书
实验方案
问题与答案
技术支持
DNMT1 Monoclonal Antibody (60B1220.1), APC

产品信息

MA5-45291

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:1,000
-

免疫组化 (IHC)

1:1,000
-

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1:10,000
-

免疫沉淀 (IP)

Assay-dependent
-

染色质免疫沉淀 实验 (ChIP)

Assay-dependent
-
产品规格

种属反应

Fish, Human, Mouse, Zebrafish

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

60B1220.1

抗原

Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1
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偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱 spectra

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

95.64mM phosphate/2.48mM MES with 0.5M EDTA

内含物

no preservative

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2931745

产品详细信息

2 µg/mL of MA5-45291 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 180kDa. It will cross-react with mouse DNMT1.

靶标信息

This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CXXC-type zinc finger protein 9; DNA (cytosine-5)-methyltransferase 1; DNA methyltransferase HsaI; DNA methyltransferase MmuI; DNA MTase HsaI; DNA MTase MmuI; Dnmt1; FLJ16293; MCMT; MGC104992

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基因别名: ADCADN; AIM; cb1075; cb983; CXXC9; ddn; dmnt1; DNMT; DNMT1; Dnmt1o; fb05h01; HSN1E; m.HsaI; m.MmuI; MCMT; Met-1; Met1; MommeD2; mta; MTase; Uim; wu:fb05h01

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UniProt ID: (Human) P26358, (Mouse) P13864

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Entrez Gene ID: (Human) 1786, (Mouse) 13433, (Zebrafish) 30430

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功能
DNA binding double-stranded DNA binding RNA binding DNA (cytosine-5-)-methyltransferase activity protein binding zinc ion binding methyl-CpG binding DNA-methyltransferase activity protein domain specific binding estrogen receptor binding histone deacetylase binding unmethylated CpG binding DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates promoter-specific chromatin binding chromatin binding catalytic activity transcription factor binding methyltransferase activity transferase activity metal ion binding DNA metabolism protein DNA methyltransferase nucleic acid metabolism protein
参与通路
negative regulation of transcription from RNA polymerase II promoter DNA methylation transcription, DNA-templated Ras protein signal transduction brain development aging response to heat response to toxic substance response to ionizing radiation maintenance of DNA methylation DNA methylation on cytosine within a CG sequence positive regulation of gene expression response to activity gene silencing covalent chromatin modification neuron differentiation response to caffeine response to estradiol response to lipopolysaccharide response to vitamin A response to testosterone cellular response to platelet-derived growth factor stimulus response to antidepressant regulation of cell proliferation DNA hypermethylation response to ethanol negative regulation of gene expression, epigenetic S-adenosylhomocysteine metabolic process S-adenosylmethioninamine metabolic process S-adenosylmethionine metabolic process positive regulation of histone H3-K4 methylation negative regulation of histone H3-K9 methylation cellular response to amino acid stimulus cellular response to lead ion cellular response to transforming growth factor beta stimulus C-5 methylation of cytosine positive regulation of methylation-dependent chromatin silencing cellular response to nerve growth factor stimulus regulation of transcription, DNA-templated metabolic process regulation of gene expression chromatin modification methylation DNA methylation on cytosine negative regulation of transcription, DNA-templated liver development lens development in camera-type eye determination of left/right symmetry retina layer formation pancreas development exocrine pancreas development intrahepatic bile duct development regulation of DNA methylation digestive tract development cartilage development histone H3-K9 methylation retina morphogenesis in camera-type eye definitive hemopoiesis Kupffer's vesicle development
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