Invitrogen

DNMT3B Polyclonal Antibody

Name: DNMT3B Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-884

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7个已发表图片

13 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

DNMT3B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DNMT3B Antibody (PA1-884) in IHC (P)

Immunohistochemistry analysis of DNA Methyltransferase 3 showing positive staining in the nucleus and cytoplasm of paraffin-treated Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blo... View More

DNMT3B Antibody in Immunohistochemistry (IHC)

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Immunogen image

Immunogen Image DNMT3B Polyclonal Antibody

产品信息

PA1-884

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2.0 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

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产品规格

种属反应

Human, Mouse

已发表种属

C. elegans, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to the residues M(1) K G D S R H L N E E E G A(14) C of mouse DNMT3b

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2277463

产品详细信息

PA1-884 detects DNA methyltransferase 3 from human and mouse tissues and cells as well as recombinant human Dnmt3. This antibody has been used to detect both recombinant and endogenous DNMT3b and recombinant DNMT3a.

PA1-884 has been successfully used in Immunohistochemistry (paraffin) and Western blot procedures. By Western blot, this antibody detects DNMT3 from P19 nuclear extracts.

The PA1-884 immunogen is a synthetic peptide corresponding to residues 1-14 of mouse DNMT3b. This sequence is 79% conserved between human and mouse. PA1-884 immunizing peptide (Cat. # PEP-117) is available for use in neutralization and control experiments.

靶标信息

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: DNA (cytosine-5)-methyltransferase 3B; DNA (cytosine-5-)-methyltransferase 3 beta; DNA methyltransferase HsaIIIB; DNA methyltransferase MmuIIIB; DNA MTase HsaIIIB; DNA MTase MmuIIIB; Dnmt3; Dnmt3b; m.MmuIIIB; MGC1090

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基因别名: DNMT3B; ICF; ICF1; M.HsaIIIB; MmuIIIB

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UniProt ID: (Human) Q9UBC3, (Mouse) O88509

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Entrez Gene ID: (Human) 1789, (Mouse) 13436

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