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DRP5B/Dynamin related protein 5B Polyclonal Antibody
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DRP5B/Dynamin related protein 5B Antibody (AS12-2634) in WB
Total proteins extracted from 5 mg leaf or flower bud of Arabidopsis thaliana tissue with SDS-loading buffer were separated on 10% SDS-PAGE using tank transfer and blotted for 1 h to nitrocellulose membrane. Blots were blocked with 2% non-fat dry milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody (affinity-purified DRP 5 B) at a dilution of 1:5,000 in TBS-T + 2%... View More
产品信息
AS12-2634
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DRP5B sequence.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Triticum aestivum
靶标信息
DRP5B encodes a plant-specific dynamin-related mechanochemical GTPase that is primarily cytosolic but is recruited to organelle surfaces, where it assembles into ring-like structures at constriction sites on the cytosolic face of the chloroplast outer envelope to drive membrane fission during plastid binary division; loss of DRP5B causes impaired constriction and enlarged, dumbbell-shaped chloroplasts, consistent with a direct role in generating constrictional force. Structurally, DRP5B is a dynamin-family protein with the canonical GTPase domain and additional dynamin-like assembly/effector regions that support self-oligomerization and nucleotide-dependent remodeling of the division ring, with GTP hydrolysis coupled to ring turnover and productive fission. Beyond chloroplasts, DRP5B also localizes to peroxisomes and contributes to peroxisome division and function, interacting with itself and with established peroxisome fission/elongation components (including FIS1 proteins, DRP3 proteins, and multiple PEX11 isoforms), supporting a model in which DRP5B acts cooperatively within a shared organelle division network to execute late-stage scission on chloroplast and peroxisome membranes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ACCUMULATION AND REPLICATION OF CHLOROPLAST 5; ARC5; AT3G19720; DRP5B; Dynamin related protein 5B; dynamin-like protein ARC5; MMB12.21
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