Invitrogen

DVL2 Polyclonal Antibody

Name: DVL2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-17471
Availability: InStock

高级验证

1 篇参考文献

查看其他12个DVL2抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (5)
高级验证 (1)

DVL2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

DVL2 Antibody (PA5-17471) in ICC/IF

Immunofluorescence analysis of DVL2 was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DVL2 Polyclonal Antibody (Product # PA5-17471) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790)... View More

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

DVL2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue panel owing to their inherent genetic constitution. Relative expression of DVL2 was observed in HCT 116 in comparison to HT-29 using DVL2 Polyclonal Antibody (Product # PA5-17471) in western blot. {RE}

Immunogen image

Immunogen Image DVL2 Polyclonal Antibody

产品信息

PA5-17471

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:100

免疫沉淀 (IP)

1:200

产品规格

种属反应

Hamster, Human, Mink, Mouse, Non-human primate, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues surrounding Ser638 of human Dvl2

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

201 µg/mL

20.1 µg

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10982147

产品详细信息

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with Dvl3.

靶标信息

Disheveled DVL2 is a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans.

仅用于科研。不用于诊断过程。未经明确授权不得转售。