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Agrisera
Deg8/protease Do-like 8 (chloroplastic) Polyclonal Antibody
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Deg8/protease Do-like 8 (chloroplastic) Antibody (AS14-2767) in WB
Total protein from Arabidopsis thaliana was extracted with buffer containing 0.2 M tris-HCl p H 6.8,10% Glycerol, 8% SDS, 20% β-mercaptoethanol, 5 M Urea. 10 µg of chlorophyll were separated on 12% SDS-PAGE using tank transfer and blotted 1.5 h to NC. Blots were blocked with 5% nonfat dry milk in TBS-T for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution ... View More
产品信息
AS14-2767
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant mature Deg8 of Arabidopsis thaliana, overexpressed in E.coli.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Nicotiana tabacum
靶标信息
Deg8 encodes a chloroplast HtrA-family, ATP-independent serine endopeptidase that is imported into the thylakoid lumen, where it associates peripherally with thylakoid membranes and functions in protein quality control during photosystem II (PSII) repair under excess light. The Deg8 polypeptide is organized as an N-terminal trypsin-like serine protease domain followed by a C-terminal PDZ domain that supports substrate recognition and/or assembly, and structural studies show Deg8 adopts an HtrA-like fold and oligomerizes (with Deg5) into higher-order complexes that position the catalytic triad for proteolysis. Biologically, Deg8 contributes to the PSII repair cycle by catalyzing endoproteolytic cleavage of photodamaged D1 (PsbA), generating characteristic N- and C-terminal fragments and thereby facilitating subsequent processive degradation and replacement of D1; loss of Deg8 (especially together with Deg5) slows D1 turnover and increases sensitivity to photoinhibition, consistent with a role in maintaining thylakoid proteostasis during high-light stress.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT5G39830; DEG PROTEASE 8; DEGP8; degradation of periplasmic proteins 8; K13H13.1; K13H13_1
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