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Invitrogen
Dinitrophenyl-KLH Polyclonal Antibody
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Dinitrophenyl-KLH Antibody (A-6430) in ICC/IF
A mixed population of trinitrophenyl-sensitized and nonsensitized bovine pulmonary artery endothelial cells that have been probed with anti-dinitrophenyl-KLH antibody (Product # A-6430), incubated with rabbit complement and then stained using the LIVE/DEAD® Reduced Biohazard Viability/Cytotoxicity Kit #1 (Product # L-7013). With this kit, the membrane-permeant SYTO® 10 dye labels the nucleic acids of live c... View More
产品信息
A-6430
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
已发表种属
Chemical,
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Dinitrophenyl-KLH
偶联物
形式
Liquid
浓度
2 mg/mL
纯化类型
purified
保存液
PBS, pH 7.2
内含物
5mM sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
DNP-KLH antibodies are used for detecting DNP-labeled molecules. Fluorescent and biotinylated anti-DNP-KLH conjugates can serve as second-step reagents for signal amplification in protein and nucleic acid detection systems, including in situ hybridization studies employing DNP-modified DNA probes. Because anti-DNP antibody binds well to DAMP (D-1552), an acidotropic reagent that can penetrate cell membranes, anti-DNP-KLH antibodies can also be used with DAMP for immunochemical localization of intracellular compartments with low pH. DNP-KLH antibodies may recognize compounds with a trinitrophenyl group, allowing the isolation of cell-surface molecules of human melanoma cells that have been labeled with trinitrobenzene sulfonic acid (TNBS).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
