Search
Agrisera
DnaK2/Heat shock protein 70-2 (HSP70-2) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
图: 1 / 1
DnaK2/Heat shock protein 70-2 (HSP70-2) Antibody (AS08-350) in WB
10 µg of total protein from total cell extract of Synechocystis PCC 6,803 wild type (WT) and mutant strain that lacks Hsp 16.6 were separated on 8% gel and blotted on nitrocellulose membrane. Filters were blocked (1 h), incubated with 1:1,000 anti-Dna K 2 antibodies (2 h) followed by incubation with 1:2,500 secondary anti-rabbit (1 h) coupled to HRP and visualization with chemiluminescent detection reagent,... View More
产品信息
AS08-350
产品规格
种属反应
Bacteria
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Full-length recombinant DnaK2 protein derived from Synechocystis PCC 6803 DnaK2 protein sequence.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 200 µL of sterile water.
Specific Species Reactivity: Synechocystis PCC 6803
靶标信息
DnaK2 encodes a canonical bacterial Hsp70-family molecular chaperone that is predominantly cytosolic in cyanobacteria and is expressed as a soluble ~70 kDa protein; like other DnaK proteins, it is organized into an N-terminal ATPase (nucleotide-binding) domain linked via a conserved flexible interdomain connector to a C-terminal substrate-binding domain capped by a helical "lid" that helps stabilize bound client polypeptides. In the cyanobacteria Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942, DnaK2 is the principal stress-responsive DnaK paralog and is essential for viability, consistent with a central role in proteostasis under both basal and stress conditions. Functionally, DnaK2 cooperates with J-domain co-chaperones and the nucleotide-exchange factor GrpE to bind exposed hydrophobic segments on nascent, misfolded, or stress-denatured proteins, using ATP-driven cycling to prevent aggregation and promote productive folding and recovery after heat and other environmental stresses. Beyond general protein quality control, DnaK2 also contributes to regulation of the heat-shock program in S. elongatus by participating in a negative-feedback loop that dampens Hik2-Rre1 two-component-system-dependent stress transcription, thereby helping restore homeostasis after thermal or redox perturbation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: chloroplast heat shock protein 70-2; cpHsc70-2; HEAT SHOCK PROTEIN 70-7; HSC70-7; HSP70-7; K9P8.5; K9P8_5
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
